猫须草HPLC指纹图谱研究  

Study on HPLC Fingerprint Analysis of Clerodendranthus spicatus

作　　者：李金雨[1] 陈淳[1] 林建忠[1] 胡维冀[1] 许文江[1] 

机构地区：[1]福建省亚热带植物研究所,福建省亚热带植物生理生化重点实验室,福建厦门361006

出　　处：《亚热带植物科学》2014年第3期197-201,共5页Subtropical Plant Science

基　　金：福建省省属公益类科研院所基本科研专项(2010R1012-4)

摘　　要：为建立猫须草药材HPLC指纹图谱分析方法,采用高效液相色谱法,以Phenomenex Synergi 4u hydro-RP250×4.60 mm为色谱柱,以甲醇-0.1%甲酸溶液为流动相梯度洗脱,检测波长254 nm,流速1.0 mL·min-1,柱温40℃。结果表明,建立的猫须草药材HPLC指纹图谱,确定了15个共有峰,各猫须草样品指纹图谱与对照指纹图谱的相似度均在0.9以上。该方法简单、准确、重复性好,为更好地控制猫须草药材质量提供有效可靠的方法。To establish a method of fingerprint analysis onClerodendranthus spicatusby HPLC, the chromatographic fingerprint was obtained by Phenomenex Synergi 4u hydro-RP 250×4.60 mm with the gradient elution solvent system composed of methanol-0.1% formic acid. The detective wavelength was 254 nm; the flow rate was 1.0 mL·min-1 and the column temperature was maintained at 40℃. The results showed that 15 co-possessing peaks were selected as the fingerprint peaks ofC. spicatus and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples ofC. spicatusand the standard fingerprint. The method with good reproducibility was simple and accurate, it could be used as a effective quality control method forC. spicatus.

关 键 词：猫须草 HPLC 指纹图谱 

分 类 号：S567[农业科学—中草药栽培] R282.5[农业科学—作物学]