青翘HPLC指纹图谱研究  

The HPLC Fingerprint of Green Forsythiae Fructus

作　　者：崔誉文 许苗苗[2] 杨黎彬[1] 杨静[1] 宋小妹[2] 崔九成[2] 

机构地区：[1]西安医学院药学院,陕西西安710021 [2]陕西中医学院药学院,陕西咸阳712046

出　　处：《南京中医药大学学报》2014年第5期478-480,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：陕西省中医管理局中医药科研项目(zy10)

摘　　要：目的建立青翘的HPLC指纹图谱。方法色谱柱:Thermo C18Column(4.6mm×250mm,5μm);柱温:30℃;流动相:乙腈-水,梯度洗脱;流速:1.0mL/min,检测波长:203nm。结果建立了青翘的HPLC指纹图谱,并标定了21个共有峰,指认了其中的3个共有峰。结论此法简便、准确、快速,为全面科学评价青翘质量提供了可靠的分析方法。OBJECTIVE To establish HPLC fingerprint map of Green Fructus forsythiae.METHODS Chromatographic Column：Thermo C18（250 mm×4.6 mm,5 μm column）; Column temperature：30 ℃; Moving phase：acetonitrile-water,solution in gradient elution; Flow rate：1.0 mL/min; The UV-detection wavelength：203 nm.RESULTS The HPLC fingerprint map of Green Fructus forsythia has been established,with 21 common peaks marked,3 of which is identified.CONCLUSION The established method is simple,accurate and rapid,which has provided reliable method for comprehensive evaluation of the quality of Green Fructus forsythia.

关 键 词：青翘 HPLC 连翘酯苷A 连翘苷 表松脂素 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]