陆地棉矮化突变体Ari1327茎尖的转录组分析  

Transcriptome Analysis of Stem Apex of an Upland Cotton Dwarf Mutant Ari1327

作　　者：安文燕 孙君灵[2] 龚文芳[2] 何守朴[2] 潘兆娥[2] 杜雄明[1,2] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430072 [2]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《植物遗传资源学报》2014年第5期1046-1052,共7页Journal of Plant Genetic Resources

基　　金：国家"973"计划(2010CB12600)

摘　　要：为了从分子水平上研究陆地棉矮秆突变体Ari1327的矮化机理,本研究以矮秆突变体Ari1327、野生型Ari971和高秆突变体Ari3697的茎尖为材料,建立3个cDNA文库,用Illumina HiSeqTM2000系统对3个材料的茎尖cDNA进行转录组测序。3个文库测序共得4.9 G数据量,拼接得到Unigene 70877个。通过矮化突变体Ari1327与野生型Ari971和高秆突变体Ari3697两个文库的差异筛选,得到13919个与矮化相关的差异表达基因,其中5406个表现上调,8513个表现下调。GO功能和KEGG通路富集发现,差异基因在植物激素信号转导途径显著富集。通过实时荧光定量PCR(qRT-PCR)验证,推测Ari1327的矮化可能与赤霉素和生长素2种激素的信号转导及互作有关。转录组测序得到的大量差异基因,为深入研究棉花的矮化机理具有重要参考价值,同时为棉花的矮化育种工作奠定了基础。To study the dwarf mechanism of Ari1327 on molecular level,three stem apex cDNA libraries of Ari1327,Ari971,and tall-culm mutant Ari3697 were constructed and sequenced using Illumina HiSeq^TM2000 system,respectively. 4. 9 G available transcriptome data were obtained,with 70877 Unigenes were spliced. After comparing the transcriptome data of Ari1327 with data of Ari971 and Ari3697,we uncovered 13919 differentially expressed genes,of which 5406 up-regulated while 8513 down-regulated in Ari1327. Gene Ontology functional enrichment and KEGG pathway analysis revealed that the differentially expressed genes enriched in plant hormone signal transduction pathway. After qRT-PCR,we speculated that the signal transduction and interaction of IAA and GA might contribute to dwarfism of Ari1327. We believed that the abundant differentially expressed genes detected by transcriptome sequencing would be beneficial for revealing the mechanism underlying cotton dwarfism which layd the foundation for breeding cotton dwarf gene type.

关 键 词：棉花(Gossypium hirsutum L.) 矮化 转录组 

分 类 号：S562[农业科学—作物学]