根际促生菌筛选及其接种剂对箭筈豌豆生长影响的研究  

Assessment of rhizobacteria strains for Vicia sativa

作　　者：马文彬[1] 姚拓[1] 王国基[1] 张玉霞[1] 荣良燕[1] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室,甘肃兰州730070

出　　处：《草业学报》2014年第5期241-248,共8页Acta Prataculturae Sinica

基　　金：国家自然基金(31360584);现代农业产业技术体系(CARS-35);甘肃省农业科技创新项目(GNCX-2012-45)资助

摘　　要：通过测定分离自箭筈豌豆和玉米根际4株细菌的固氮酶活性、溶磷量及分泌生长素能力,将其制成植物根际接种剂,并结合半固体培养试验测定接种剂对箭筈豌豆生长的影响。结果表明,菌株J3-1、J1-15和Y16具备溶磷和分泌生长素能力,J1-15的溶磷能力最强,为548.9mg/L,Y16分泌生长素能力最好,达17.8μg/mL,且菌株Y16具较强固氮能力,J3固氮酶活性为366.51C2H4nmol/(mL·h)。与对照组相比,单一菌株制备的接种剂处理(Y16)可使箭筈豌豆地上生物量、地下生物量分别显著增加104.5%和254.1%(P<0.05),复合接种剂处理F(J3-1+J1-15+Y16+J3)使箭筈豌豆地上和地下生物量分别增加76.1%和192.3%。综合各指标,复合接种剂处理效果明显优于单一接种剂,处理F(J3-1+J1-15+Y16+J3)可使箭筈豌豆株高、根长、根表面积、根体积、根系活力,分别较对照增加29.4%,70.0%,174.0%,194.6%,38.3%。这主要是由于菌种间的互作效应造成的。To investigate the effect of rhizobacteria inoculants on the growth ofViciasativa,four rhizobacteria strains （J3-1,J1-15,Y16 and J3）were assessed for potentially useful characteristics with the potential to en-hance the growth and yield ofV.sativa;high nitrogenase activity,high phosphate solubilisation and ability to secrete 3-indoleacetic acid （IAA）into the rhizosphere.Strains J3-1,J1-15 and Y16 had the ability to solubilize phosphate and secrete IAA.J1-15,Y16,J3-1 had the strongest phosphate solubilizing capacity （548.9 mg/L）, IAA secreting ability （17.8μg/mL）,and nitrogenase activity （366.51 C2 H4 nmol/mL＆#183;h）,respectively.Y16 increased the aboveground biomass and underground biomass ofV.sativaby 104.5%and 254.1%,respective-ly,compared to the non-inoculated control.An inoculant mixture of all four strains increased the aboveground biomass and underground biomass ofV.sativaby 76.1% and 192.3%,respectively,relative to the control. This resulted from an increase in plant height,root length,root surface area,root volume and root activity of 29.4%,70.0%,174.0%,194.6% and 38.3%,respectively.The results suggest that inoculants including mixtures of rhizobacteria strains may have significant benefits forV.sativacultivation.

关 键 词：促生菌(PGPR) 接种剂 箭筈豌豆 生长 根系 

分 类 号：S551.9[农业科学—作物学]