东祁连山高寒草地几株醉马草内生细菌的生物功能评价及鉴定  

Identification and determination of biological functions of endophytic bacteria fromAchnatherum inebrians in Alpine grassland of East Qilian Mountains

作　　者：杨成德 王颖 王玉琴 姚玉玲 薛莉 徐长林 陈秀蓉 

机构地区：[1]草业生态系统教育部重点实验室甘肃农业大学草业学院甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业学报》2014年第5期249-255,共7页Acta Prataculturae Sinica

基　　金：草业生态系统教育部重点实验室(甘肃农业大学)开放课题项目(No.CYzs-2011011)资助

摘　　要：采用表面消毒划线法从醉马草分离获得6株内生细菌,分别命名为261MG1、261MG2、261MG3、261MG4、261MY5和261MY6,并对其进行了生物功能测定和鉴定。结果表明,有5株内生细菌对马铃薯炭疽病菌和马铃薯坏疽病菌有较好拮抗作用,有4株对马铃薯枯萎病菌有较好拮抗作用,且261MG2、261MG3、261MG4和261MY6对3种病原菌均有较好拮抗作用;除261MG4外均有固氮和产吲哚乙酸能力;通过培养性状和形态特征,结合16S rDNA序列相似性分析,将261MG1、261MG2、261MG3、261MY6、261MG4和261MY5分别鉴定为枯草芽孢杆菌Bacillus subtilis、Bacillus axarquiensis、莫海威芽孢杆菌Bacillus mojavensis、解淀粉芽孢杆菌Bacillus amyloliquefaciens、耐盐短杆菌Brevibacterium halotolerans和棒形杆菌Clavibacter sp.。该研究结果为醉马草内生细菌进一步开发利用提供了依据。6 endophytic bacteria of Achnatheruminebrians collected from Alpine grassland on East Qilian Mountains were isolated using a surface disinfection method,and named 261MG1,261MG2,261MG3, 261MG4,261MY5 and 261MY6,respectively.Of these,5 strains had strong antifungal activity againstColle-totrichumcoccodes and Phomafoveata,and 4 strains were active against Fusariumavenaceum.In addition, 261MG2,261MG3,261MG4 and 261MY6 had inhibitory activity against 3 other pathogens.Except for 261MG4,the endophytic bacteria had the nitrogen-fixing ability and IAA （indole acetic acid）secreting.Based on the culture characteristics,morphological characteristics and 16S rDNA gene sequence analysis,261MG1, 2 6 1 MG2 ,2 6 1 MG3 ,2 6 1 MY6 ,2 6 1 MG4 and 2 6 1 MY5 were identified as Bacillussubtilis,Bacillusaxarquien-sis,Bacillusmojavensis,Bacillusamyloliquefaciens,Brevibacteriummichiganensis,and Clavibactersp .,re-spectively.The study provides the evidence for the existence of endophytic bacteria of A.inebriansin East Qil-ian Mountains Alpine grassland,and the initial exploration of their identity and properties.

关 键 词：醉马草 内生细菌 生物功能 鉴定 

分 类 号：S543.9[农业科学—作物学] S476.1