鲆鲽类主要病原菌抗血清的制备及应用  

The preparation and application of inactivated vaccine and its antiserum from eight common pathogens of Flatfish

作　　者：甘玲玲[1,2] 王蔚芳[1] 高淳仁[1] 雷霁霖[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛市海水鱼类种子工程与生物技术重点实验室,山东青岛266071 [2]四川省富顺县水产渔政局,四川富顺643200

出　　处：《中国工程科学》2014年第9期21-25,共5页Strategic Study of CAE

基　　金：现代农业产业技术体系建设专项资金(CARS-50)

摘　　要：用甲醛灭活8种鲆鲽类主要病原菌,鳗弧菌(Vibrio anguillarum)、创伤弧菌(Vibrio vulnificus)、哈维弧菌(Vibrio harveyi)、溶藻弧菌(Vibrio alginolyticus)、嗜水气单胞菌(Aeromonas hydrophila)、豚鼠气单胞菌(Aeromonas caviae)、迟缓爱德华菌(Edwardsiella tarda)、鮰爱德华菌(Edwardsiella ictaluri),制备成灭活疫苗后肌肉注射大菱鲆获得各病原菌抗血清。结果显示,抗鳗弧菌血清、抗哈维弧菌血清、抗豚鼠气单胞菌血清、抗鮰爱德华菌效价为1:6 400,抗创伤弧菌血清、抗溶藻弧菌血清、抗嗜水气单胞菌血清效价为1:12 800,抗迟缓爱德华菌血清效价为1:102 400。应用ELISA分析各抗血清与8种病原菌及迟缓爱德华菌蛋白的免疫交叉反应,结果显示,各病原菌与其本身的抗血清反应最为强烈,而与其他抗血清间有程度不等的交叉反应;菌蛋白与其相应的菌抗血清反应最为强烈,与其他病原菌抗血清反应较弱。本研究所制备的病原菌抗血清效价较高,且具有特异性,可应用于鲆鲽类病原菌的检测。In this study,formal dehyde was used to inactivate Edwardsiella tarda,Vibrio anguillarum, Vibrio harveyi, Vibrio vulnificus, Vibrio alginolyticus, Aeromonas caviae, Edwardsiella ictaluri,Aeromonas hydrophila,in order to preparethe inactivated vaccine. Then vaccine was used to immune turbot by intramuscular injection. In this way ,we got the antise-rum of all bacterium above. Enzyme-linked immunosorbent assay was used to determine the content of antibody in serum. The antibody can be detect after four week in the immune,and the level of antibody is research highest in the tenth week which is inoculated inactivated vac-cine of Edwardsiella tarda,and the highest titer of other vaccines are 1：102 400,1：6 400, 1：6 400,1：12 800,1：12 800,1：6400,1：6 400,1：12 800. The results of cross reaction of the bacteria and their anti-serum shows that the effect is most intense when the bacteria react with its own antiserum,and it is very weak when the bacteria react with other antiserum.

关 键 词：鲆鲽类 疫苗 抗血清 病原菌 

分 类 号：S941[农业科学—水产养殖]