刘静  

Effect of Ca2＋ on CcRIP II-2 Gene Transformation of NingyangJujube

作　　者：黄艳艳[1] 罗磊[1] 牛庆霖[1,2] 刘峰[2] 翁曼丽[3] 刘静[1] 冯殿齐[1] 

机构地区：[1]泰安市泰山林业科学研究院,山东泰安271000 [2]山东农业大学,山东泰安271018 [3]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《山东农业大学学报（自然科学版）》2014年第4期481-488,494,共9页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：抗枣疯病植原体（MLO）基因转化项目

摘　　要：本研究应用农杆菌介导的方法,采用L9（34）正交试验设计,对影响宁阳大枣基因转化的各项因子进行了分析比较,并着重探索了Ca2＋浓度对侵染转化的影响.结果表明：侵染效率最高的组合为1/2 侵染液浓度,20 min 侵染时间和0 mg/L Ca2＋浓度.Ca2＋浓度在提高宁阳大枣基因转化效率方面没有明显促进效果.Ca2＋浓度仅对平均分化芽数影响较大,浓度为0 mg/L 时,平均分化芽数最多.本试验共获得27 株转化苗,试验转化效率为33.33%.In this study, the concentration effect of Ca2＋ on transformation of RIP gene （Ribosome Inactivating Protein Gene）cloned from Cinnamomum camphora to Ningyang Jujube was investigated in detail with L9（34） orthogonal design, throughthe analysis of factors which affected the transformation. The results showed that the concentration of Ca2＋ had no crucialeffect on transformation and the best combination was the bacterium solution with 0.4-0.6 OD value was doublediluted,20-minutes infection and without Ca2＋. 27 transformation seedlings were obtained and the transformation rate was33.33%.

关 键 词：农杆菌介导法 香樟核糖体失活蛋白基因 枣疯病 Ca2+浓度 

分 类 号：S722.3[农业科学—林木遗传育种]