利用TRAP标记分析8个福瑞鲤家系的遗传多样性  

Genetic diversity of eight FFRC strain common carp families revealed by target region amplified polymorphism markers

作　　者：丁敬波[1] 董在杰[1,2] 朱文彬[2] 苏胜彦[2] 公翠萍 阎明信 

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心 农业部淡水渔业和种质资源利用重点实验室,江苏无锡214081

出　　处：《江苏农业学报》2014年第5期1077-1086,共10页Jiangsu Journal of Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金项目(CARS-46);国家“十二五”科技支撑计划项目(2012BAD26B02)

摘　　要：为了研究福瑞鲤不同家系的遗传多样性,利用TRAP分子标记对生长速度有差异的家系（4个生长速度快,4个生长速度慢）进行了遗传分析。结果显示：8个家系的多态性条带数在9.41至12.00之间,多态性比率范围为52.04%～66.02%,多态信息含量为0.1853～0.2430, Nei 遗传多样指数和 Shannon 信息指数分别为0.1875～0.2410和0.2780～0.3510,其中家系85^#的各遗传参数值最高,而家系74#的最低。家系间的遗传分化指数（Fst）及分子变异方差（AMOVA）分析结果显示,除了家系56^#与92^#（Fst=0.0516）及51^#与89^#（Fst=0.0956）这2对家系间处于中等程度的遗传分化（0.05≤Fst≤0.15）之外,其他家系间遗传分化水平均较低（Fst〈0.05）,群体的总遗传变异主要来源于家系内个体间的遗传差异,其方差分量的贡献率达98.3%。遗传距离分析结果表明,家系26^#与92#的遗传距离最大,家系85^#与89^#的遗传距离最小；基于此的UPGMA聚类分析结果显示,家系56^#与74^#以及家系85^#与89^#的亲缘关系比较近,聚类在同一分支上,而其他家系亲缘关系相对较远。研究结果说明8个福瑞鲤家系内遗传变异较大,遗传多样性水平较高,具有一定的育种潜力,可扩大福瑞鲤的选育空间。To reveal the diversity of differently developed FFRC strain common carp （ Cyprinus carpio） , target region amplified polymorphism（TRAP） molecular markers were used to analyze 8 families （four families grow fast and other four grow slowly）. The results show that the number of polymorphism bands of the eight families ranged from 9. 41 to 12. 00, the polymorphism percentage ranged from 52. 04% to 66. 02%, the polymorphism information content （ PIC ） ranged from 0. 185 3 to 0. 243 0, and the Nei’s genetic diversity and Shannon’s indexes ranged from 0. 187 5 to 0. 241 0 and from 0. 278 0～nbsp;to 0. 351 0, respectively. Family 85^# had the highest genetic parameter values, while 74^# had the lowest. Pairwise Fst（genetic differentiation index） and AMOVA a-nalysis showed that except for 56# and 92#（Fst=0.051 6） as well as 51^#and 89^#（Fst=0. 095 6） in moderate differen-tiation（0. 05≤Fst≤0. 15）, the rest of the families were in＆amp;nbsp;lower genetic differentiation （Fs〈0. 05）. The majority of genetic variability was within family （98. 3%）, rather than among family （1. 7%）. The genetic distance between 26^#and 92^# was farthest while the genetic distance between 85^#and 89^# was the lowest. The UPGMA phylogenetic tree of the eight families constructed based on Nei’s genetic distance clustered 56^# and 74^# together, and 85^#and 89^#. The findings suggested that the eight families had a large selection potential due to the great genet-ic variation and high level of genetic diversity.

关 键 词：TRAP分子标记 福瑞鲤 家系 遗传多样性 遗传分化 

分 类 号：S965.116[农业科学—水产养殖]