棉花烟雾吸入性急性肺损伤大鼠模型的建立  

The establishment of cotton smoke inhalation-induced acute lung injury model in rats

作　　者：韩志海[1] 段蕴铀[1] 姜毅 王晓阳[1] 方庭正[1] 黄燕[1] 

机构地区：[1]海军总医院呼吸内科,北京100048 [2]沈阳军区政治部门诊部,辽宁沈阳110032

出　　处：《转化医学杂志》2014年第5期274-277,共4页Translational Medicine Journal

基　　金：全军医学科研课题(CHJ13J013)

摘　　要：目的建立大鼠烟雾吸入性急性肺损伤动物模型。方法 24只雄性SD大鼠随机分成对照组及3h、6h、24h组,每组6只。对照组无烟雾吸入,3h、6h、24h组分别在烟雾吸入3、6、24 h后行血气分析及炎症因子、组织学检查。结果烟雾吸入组大鼠血气分析示氧合指数小于300,符合急性肺损伤诊断标准,炎症细胞因子较对照组明显升高(P<0.05);组织学检查示肺内出现肺水肿,炎症细胞浸润明显。结论通过烟雾发生装置给予大鼠吸入一定时间棉花制造的烟雾,可以成功制作烟雾吸入急性肺损伤动物模型。Objective To establish an acute lung injury model by cotton smoke inhalation in rats.Methods Twenty-four male SD rats were randomly allocated into control group, after smoke inhalation 3h, 6h and 24h group.The changes in blood gas analysis, inflammatory mediators such as interleukin 8 (IL-8) were monitored in every group.Histopathology of lung in rats were observed. Results Compared with control group.PaO2 and PaCO2 were significantly decreased in every smoke inhalation group(P<0.05).The increased of inflammatory mediators clew that lung injury were occurred after smoke inhalation in rats.Obvious edema, alveolar atrophy and inflammatory infiltra-tion were found in smoke inhalation rats.Conclusion An acute lung injury model by cotton smoke inhalation in rats has been successfully established.

关 键 词：急性肺损伤 烟雾吸入 棉花 

分 类 号：R563[医药卫生—呼吸系统] R-332[医药卫生—内科学]