HPLC法测定千金子不同极性部位中二萜酯类化合物含量  

Comparison of Content of Diterpene Esters from Different Polar Fractions in Euphorbiae Semen by HPLC Method

作　　者：张超[1] 朱娟娟[1] 王英姿[2] 孙秀梅[1] 

机构地区：[1]山东中医药大学,山东济南250355 [2]北京中医药大学,北京100102

出　　处：《山东中医药大学学报》2014年第5期485-488,共4页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81274082);教育部高等学校博士学科点专项(编号:20110013120007);北京市自然科学基金项目(编号:7122093)

摘　　要：目的:考察千金子素L8、千金子素L1、千金子素L2、千金子素L3四种化合物在千金子不同极性部位的分布情况。方法:用HPLC测定,色谱柱为Inertsil ODS-3(250 mm×4.6 mm,5μm),流动相为甲醇-水(75∶25),流速1.0 mL·min-1,柱温25℃;检测波长275 nm。结果:4个二萜酯类化合物在石油醚部位中含量最高,在乙酸乙酯和正丁醇部位中较少,在剩余水部位基本检测不到二萜酯类化合物。结论:该方法准确简便,重现性好,可用于比较千金子不同极性部位二萜酯类化合物的分布情况。Objective：To study the distribution of euphorbia factor L8,euphorbia factor L1,euphorbia factor L2 and euphorbia factor L3 in the different polar fractions of Euphorbiae semen. Methods：Using HPLC method determine the content of 4 ingredients. The column is performed on Inertsil ODS-3（250 mm×4.6 mm,5 μm）,with mobile phase for methanol and water（75∶25）,velocity 1.0 mL·min-1,column temperature 25 ℃,275 nm wavelength detection. Results：The content of diterpene esters was the highest in the petroleum ether fraction of Euphorbiae semen. The content of diterpene esters was lower in the fractions of acetic ether and n-butanol. In the remaining part of water,the diterpene esters were undetected. Conclusion：The method is simple,accurate and well reproducibile,so it can be used to compare the content of diterpene esters from different polar fractions in Euphorbiae semen.

关 键 词：千金子 不同极性部位 含量测定 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]