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出 处:《海洋渔业》2014年第5期418-423,共6页Marine Fisheries
基 金:广东省科技计划项目(2012A020602048);广东省海洋渔业科技推广专项项目(201101I02)
摘 要:报道了长鳍裸颊鲷(Lethrinus erythropterus)外周血细胞的显微结构。血涂片经过WRIGHT氏染色,在Olympus BX51显微镜下观察,可鉴别出红细胞、淋巴细胞、嗜中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞、血栓细胞和单核细胞等7种类型的血细胞。红细胞数量最多,多为椭圆形,具有圆核;在白细胞中,数量多少依次是血栓细胞、淋巴细胞、嗜中性粒细胞和单核细胞,嗜碱性粒细胞在外周血液中很罕见。血栓细胞体积最小,嗜中性粒细胞最大。淋巴细胞包括小淋巴细胞、中淋巴细胞和大淋巴细胞3种。Microstructure of peripheral blood cells in Lethrinus erythropterus were morphologically described to accumulate basic data for blood morphological features of L.erythropterus,provide scientific and theoretical basis for breeding and disease control in the future,and provide references for fish physiology and immunology research of L.erythropterus.The blood of L.erythropterus were stained with Wright.Morphological characteristics of blood cells were evaluated with Olympus-BX51 digital microphotography.On the stained smears,seven major types of blood cells were recognized:erythrocyte,neutrophil,eosinophil,basophil,lymphocyte,monocyte and thrombocyte.Among these ceils,erythrocyte was the most abundant and had round nucleus.In quantity,thrombocyte was the largest,lymphocyte the second,then neutrophilic granulocyte and monocyte,basophilic granulocyte the least.Thrombocyte was the highest,accounting for 62.53% of the total number of white blood cells.Basophilic granulocyte accounted for only 0.93% of white blood cells.Thrombocyte had the smallest size among leucocytes,while monocyte the largest.Lymphocytes could be divided into three types:small lymphocyte,medium lymphocyte and big lymphocyte.
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