C_(17)、K培养基在小麦花药培养脱分化中的应用效果研究  

Application of C_(17) and K Culture Media in Dedifferentiation of Wheat Anther Culture

作　　者：杨雪[1] 邢文会[1] 刘春雷[1] 张丽琴[1] 王丁[1] 王世杰[1] 常慧萍[1] 韩鸿鹏[1] 

机构地区：[1]河南教育学院生命科学系,河南郑州450046

出　　处：《河南农业科学》2014年第9期28-30,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31071413);河南省科技攻关重点项目(122102110189);河南省自然科学研究项目(2011B210002);河南省基础与前沿技术研究项目(132300410457);河南教育学院青年课题(20090103)

摘　　要：为提高小麦花药培养的诱导率,筛选出更适合河南小麦花药培养的脱分化培养基,采用128个小麦杂交组合,将其花药接种于C17和K培养基上,对2种培养基在小麦花药培养脱分化过程中的效果进行比较,并以K培养基为基本培养基,分别添加0、0.25、0.5、1.0、1.5、2.0mg/L KT,探究培养基中KT浓度对小麦花药脱分化的影响。结果表明,K培养基愈伤组织诱导率(4.66%)明显高于C17培养基(2.45%)(P<0.05),两者变异系数表现为C17(0.56)>K(0.22),说明K培养基诱导小麦花药愈伤组织的稳定性较好。在K培养基中加入0.25mg/L的KT,小麦花药脱分化效果显著高于其他KT质量浓度(P<0.01),且稳定性最好。The purpose of this study was to increase the induction rate of wheat anther culture, and to screen the culture medium suitable for the dedifferentiation of wheat anther culture in Henan province.The anthers of 128 wheat crosses were inoculated on C17 medium and K medium, and the effects of the two media were compared.Taking K as the basic culture medium,it was added with 0,0.25,0.5,1.0,1.5,2.0 mg/L KT to explore the effect of KT concentration on the dedifferentiation of wheat anther culture.The results showed that the rate of callus induction was significantly higher on K culture medium（4.66%）than on C17 culture medium（2.45%）（P〈0.05）.In addition,the stability of wheat callus induction was also better on K culture medium than on C17 culture medium according to their variation coefficients（0.56 for C17 and 0.22 for K）. Adding 0.25 mg/L KT in the culture medium had the best effect in wheat anther differentiation （P〈0.0 1 ）and the highest stability.

关 键 词：C17培养基 K培养基 小麦花药培养 愈伤组织 诱导率 

分 类 号：S512.1[农业科学—作物学]