利用基因芯片筛选辐射损伤早期生物标志物  

Screening of biomarker for radiation injury using microassay

作　　者：王治东[1] 沈丽萍[1] 林仲武[2] 张学清[1] 李孝鑫 陈英[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]军事医学科学院科技部,北京100850

出　　处：《辐射研究与辐射工艺学报》2014年第5期35-41,共7页Journal of Radiation Research and Radiation Processing

摘　　要：以人外周血全血为研究对象,筛选本底值均一的辐射敏感基因,寻找辐射损伤早期的生物标志物。采集3名健康成人外周血,采用?-射线对其分别进行0、0.75、2和6 Gy照射,照射后2 h提取全血总RNA,利用全基因组芯片筛选差异表达基因,并分析差异表达基因在3个个体中的本底值。结果显示,共筛选到15个具有相对均一本底值的辐射诱导基因,这些基因照射后的变化具有一定的剂量依赖性:ISG20L1、TRAF4和TRIAP1等3个基因在0.75、2和6 Gy 3个剂量照射后2 h均上调2倍以上;LOC345537、POLH、SPIB、TNFSF9和TCL1A等5个基因在2 Gy和6 Gy照射后2 h变化2倍以上,其中TLC1A为下调表达,其余4个上调表达;C12orf5、DDB2、GADD45A、MADCAM1和RPS27L仅在6 Gy照射后2 h上调2倍以上。To screen radiation-sensitive genes with uniform background levels in human peripheral blood, which can be used as biomarkers for radiation injury, peripheral blood samples from three healthy adults were exposed to gamma-rays with dose of 0, 0.75, 2 and 6 Gy. The total RNA was extracted from the whole blood and differential gene expression profiling was analyzed using microarray at 2 h after irradiation. The results showed that 15 differential expressive genes with a relatively uniform background were found： ISG20L1, TRAF4 and TRIAP1 were up-regulated over 2-fold at 2 h after 0.75, 2 and 6 Gy irradiation; LOC345537, POLH, SPIB and TNFSF9 were up-regulated over 2-fold, and TCL1A was down-regulated over 2-fold at 2 h after 2 and 6 Gy irradiation; C12orf5, DDB2, GADD45A, MADCAM1 and RPS27L were up-regulated over 2-fold at 2 h after 6 Gy irradiation.

关 键 词：电离辐射 基因芯片 辐射敏感基因 生物标志物 

分 类 号：TL71[核科学技术—辐射防护及环境保护]