纸基微孔阵列芯片比色法检测乳酸脱氢酶  

Colorimetric Determination of Lactate Dehydrogenase on Paper-Based Microwell Arrays Microfluidic Device

作　　者：张慧妍[1,2] 张珍[1] 吉邢虎[1] 何治柯[1] 

机构地区：[1]生物医学分析化学教育部重点实验室,武汉大学化学与分子科学学院,武汉430072 [2]核工业北京化工冶金研究院,北京101149

出　　处：《分析化学》2014年第9期1276-1280,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.21205089);教育部高等学校博士学科点专项科研基金(No.20120141120036)资助项目~~

摘　　要：利用纸基微芯片便捷、直观的优势,采用吩嗪二甲酯硫酸盐（PMS）/氯化硝基四氮唑蓝（NBT）显色体系,借助凝胶成像仪和普通照相机两种成像方式,建立了纸基微孔阵列芯片比色法检测乳酸脱氢酶（LDH）的方法。在最佳实验条件下,显色强度与LDH浓度呈线性相关。采用凝胶成像仪检测时,线性范围为10~150 U/L,检出限（3σ）为9.44 U/L（n=18）。采用照相法获得的线性范围为15~150 U/L,检出限（3σ）为12.36 U/L（n=18）。实验表明,人血清白蛋白（HSA）对显色结果具有增强作用,探讨了HSA的增色作用,并以HSA为增强试剂得到工作曲线。基于纸基微孔阵列芯片的LDH活性测定方法具有操作简单、结果直观可见、灵敏度高等优点,对于脱氢酶类的便捷检测有一定参考价值,可望在生物医疗检测领域获得应用。A low-cost, simple, sensitive detection method of lactate dehydrogense （LDH） was developed on paper-based microwell arrays microfluidic device. The phenazine methyl sulfate/nitrotetrazolium blue chloride （PMS/NBT） detection system was used for LDH detection and the colorimetric results were recorded by both Gel Documentation System and a common camera. Under the optimized conditions, the colorimetric intensity showed a linear correlation to the activity of LDH in the range of 10 to 150 U/L with a limit of detection （LOD） of 9.44 U/L （30σ） by Gel Documentation System; and the linear range was 15-150 U/L by camera with a LOD of 12.36 U/L （30σ）. Foremost, it was found that human serum albumin （HSA） had an effect on the colorimetric enhancement in this detection system. This low-cost, portable paper-based analytical platform could be suitable for the application in the point-of-care with high sensitivity and reproducibility.

关 键 词：乳酸脱氢酶 人血清白蛋白 纸基微孔阵列芯片 比色法 

分 类 号：O657.3[理学—分析化学] O629.8[理学—化学]