季铵盐化纳米金刚石-肌红蛋白修饰电极的电化学行为  

Electrochemical Characterizations of Quaternarized Nanodiamond-Myoglobin Modified Electrode

作　　者：马晓玲[1] 丁承君[1] 张璞[1] 郭玮[1] 罗红霞[1] 

机构地区：[1]中国人民大学化学系,北京100872

出　　处：《分析化学》2014年第9期1332-1337,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21075136)资助~~

摘　　要：通过一系列的化学反应对纳米金刚石(ND)表面进行修饰,成功制备了季铵盐化纳米金刚石:ND-CO-NH-CH2-CH2-N(CH3)3+·I-(QAS-ND),通过FT-IR、元素分析、电化学等手段对目标产物QAS-ND进行了表征。将肌红蛋白(Mb)与QAS-ND混合液滴加在玻碳(GC)电极表面,制备QAS-ND/Mb/GC修饰电极。在0.1 mol/L磷酸盐缓冲溶液(pH 7.0)中,固定在膜内的Mb表现出良好的直接电化学性质,并显示了很好的稳定性。同时,探讨了此修饰电极表面固定的Mb对H2O2的催化还原,结果表明,此修饰电极可作为H2O2生物传感器,实现对H2O2的快速、准确检测,检出限为3.5μmol/L(S/N=3)。Through a series of chemical reactions, a kind of quaternary ammonium salt derivative of nanodiamond, ND-CO-NH-CH2-CH2-N（CH3）3^＋·I^-（QAS-ND）, was obtained , which was confirmed by FTIR, element analysis experiment and the electrochemistry measurements. Mixed myoglobin （Mb） and QAS- ND solution was dropped on the surface of the glassy carbon （ GC ） electrode to prepare QAS-ND/Mb/GC modified electrode. In 0.1 mol/L phosphate buffer solution （PBS） （ pH 7.0）, Mb in the membrane exhibited direct electrochemical properties and showed good stability. The electrocatalytic property of the modified electrode toward H202 was investigated, the results showed that the modified electrode could be used as the H202 biosensor to achieve fast, accurate detection of H202, with a detection limit of 3.5 mmol/L （S/N=3）.

关 键 词：季铵盐化纳米金刚石 肌红蛋白 化学修饰电极 循环伏安法 过氧化氢 

分 类 号：O646.5[理学—物理化学]