碱溶分光光度法测定植硅体碳含量  

Determination of Phytolith-occluded Carbon Content Using Alkali Dissolution-Spectrophotometry

作　　者：杨杰[1] 李永夫[1,2] 黄张婷[1] 姜培坤[1,2] 项婷婷[1] 应雨骐[1] 

机构地区：[1]浙江农林大学,临安311300 [2]浙江省森林生态系统碳循环与固碳减排重点实验室,临安311300

出　　处：《分析化学》2014年第9期1389-1390,共2页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.31270667);浙江省自然科学基金重点项目(No.LZ12C16003);浙江省林业碳汇与计量科技创新团队(No.2010R50030)共同资助~~

摘　　要：1引言近年来,植硅体碳作为一种了解全球气候变化潜力的稳定型有机碳已被科学工作者广泛研究[1,2]。为进一步对植硅体碳进行深入研究,开发出快速准确检验植硅体中有机碳含量的新方法变得尤为迫切。目前,植硅体碳的测定方法主要有Elementar Vario MAX CNS分析仪法和酸溶法[3,4],前者需要高温条件及特定仪器,难于适应大批样品低损耗、快速经济检测的需求;后者存在环境污染的风险,同时也对操作人员具有一定的安全隐患,本实验建立了强碱快速溶解植硅体，重铬酸钾一硫酸溶液氧化分光光度法定量测定植硅体碳的方法，扩大了检测限度，降低了分析成本。A method to determine phytolith-occluded carbon （ PhytOC ） was explored. Sodium hydroxide solution was used to dissolve the silicon compound（ SiO2 · nH2O） which occluding on the carbon, and then potassium dichromate-sulphuric acid solution was used to oxidize the released organic carbon. The concentration of Gr^3＋ produced from the reaction was measured by spectrophotometry with its absorbance at 590 nm wavelength. The organic carbon concentration was calculated based on the amounts of potassium dichromate consumed. This analytical method was verified well accuracy and repeatability by running standard addition and recovery experiments. It has the advantages of low carbon loss, easy to operate, low cost ; and hardly any influence to body health, as compared with acid dissolution-Elementar Vario MAX CN method. The results obtained from this method have a good correlation with these determined by Elementar Vario MAX CN （Germany）.

关 键 词：分光光度法测定 有机碳含量 植硅体 碱溶 全球气候变化 科学工作者 VARIO 测定方法 

分 类 号：O657.3[理学—分析化学]