异氟烷对新生大鼠皮质星形胶质细胞形态和幼鼠行为学的影响  

The effects of prolonged exposure to isoflurane on the morphology of the astrocytes in the cortex and the memory and learning ability of neonate rats

作　　者：钟敏[1] 李志鹏[1] 柳垂亮 林明[1] 肖建斌[1] 

机构地区：[1]广东省中医院麻醉科,广州510120 [2]广东省佛山市禅城区中心医院麻醉科,528031

出　　处：《广东医学》2014年第17期2659-2661,共3页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(编号:S2011010004786)

摘　　要：目的探讨长时间吸入低浓度异氟烷对新生大鼠皮质星形胶质细胞的形态及幼鼠空间学习和记忆能力的影响。方法 43只新生Wisrar大鼠随机分成两组,I组吸入1.1%的异氟烷6 h,C组吸入压缩空气和氧气的混合气体6 h,两组均维持吸入氧浓度为30%。在吸入麻醉后12、24、72、168 h两组分别取5只幼鼠,灌注取脑后用免疫荧光法标定星形胶质细胞,用Image J软件分析星形胶质细胞形态学的改变。在吸入异氟烷后6周,记录剩余幼鼠的存活率并将剩下的动物进行Morris水迷宫实验测试其空间学习和记忆能力。结果与C组相比,I组12 h和24 h激活星形胶质细胞的数目减少(P<0.05),12 h时抑制状态的星形胶质细胞的数目明显上升(P<0.05),其余时间点两组之间星形胶质细胞数目差异无统计学意义。两组幼鼠定位航行实验和空间探索实验结果差异无统计学意义。结论长时间吸入低浓度异氟烷会导致星形胶质细胞形态的暂时性改变,但是对于幼鼠成年后的认知功能无明显影响。Objective To evaluate the effects of prolonged exposure to isoflurane on the morphology of the astrocytes in the cortex and the memory and learning ability of neonate rats. Methods Seven- days- old littermates（ n =43） of Wistar rats were randomly assigned to isoflurane group（ Group I） and control group（ Group C）. Both groups experienced either 6- hour exposure to 1. 1% isoflurane or air with 30% oxygen. Five rats in each group were euthanized 12 h,24 h,3 d and 7 d after exposure. Brain sections were stained with glial fibrillary acidic protein markers. Astrocytes was classified and quantified in the cortex according to the morphology by the Image J programme. Spatial memory and learning ability were examined in the Morris Water Maze. Results The activated astrocytes were significantly reduced in subjects exposed to isoflurane at 12 h and 24 h in the cortex region（ P〈 0. 05）,while the depressed astrocytes were increase at 12h（ P〈 0. 05）. There was no statistical difference between the 2 groups according to the spatial reference memory and learning ability. Conclusion Prolonged isoflurane exposure of neonate rats evokes an transiently decrease of activation of the astrocytes and increase of depressed astrocytes,however does not impair the juvenile memory and learning ability.

关 键 词：异氟烷 星形胶质细胞 胶质纤维酸性蛋白 水迷宫 

分 类 号：R285.5[医药卫生—中药学]