不同频率的周期性压力对兔软骨细胞影响的生物学研究  

Effect and Mechanism of Cyclical Pressures of Different Frequencies on Rabbit Chondrocytes

作　　者：黄正[1] 杨松滨[1] 刘益杰[1] 褚立希[1] 冯伟[1] 陈世宣 

机构地区：[1]上海中医药大学康复医学院,上海201203

出　　处：《河北医学》2014年第12期1941-1944,共4页Hebei Medicine

基　　金：国家自然科学基金项目;编号:(81173357);上海市浦东新区卫生系统重点学科群建设资助项目;编号:(PWZxkq2011-01);上海市医学重点专科项目;编号:(ZK2012a25)

摘　　要：目的:通过观察不同频率的周期性压力对兔软骨细胞增殖及整合素α1β1分泌量的影响,研究不同频率的力学刺激对软骨细胞产生的生物学影响。方法:取兔四肢关节的软骨细胞,在体外进行培养。表型稳定的第3代细胞进行研究,设置常压培养A组和周期性压力B组,并将B组在压力为172kPa按照加压频率分组,加压频率B1组为0.2Hz,B两组0.5Hz,B3组1Hz,B4组2Hz,B5组5Hz。B组每天干预2h,连续干预3d。检测A和B组软骨细胞增殖情况和整合素α1β1的含量。结果:OD值A组为1.08±0.13,B1组为1.19±0.11,B两组为1.23±0.08,B3组为1.35±0.06,B4组为1.45±0.09,B5组为1.37±0.12。加压各组与对照组比较OD值升高,差异有统计学意义。B组各组间差异有统计学意义,峰值出现在B4组。A组整合素α1β1水平为3.02±0.26pg/mL,B1组为3.28±0.19pg/mL,B两组为3.43±0.11pg/mL,B3组为3.77±0.24pg/mL,B4组为4.01±0.09pg/mL,B5组为3.94±0.13pg/mL。加压各组与对照组比较整合素α1β1含量均有升高,差异有统计学意义差异(P<0.05)。B组各组间整合素差异有统计学意义,峰值出现在B4组。结论:相同大小(172kPa)不同频率的周期性压力刺激兔软骨细胞,均能促进软骨细胞增殖和增加整合素含量的作用,但并非频率越高作用越强。本实验中以2Hz频率的周期性压力作用最强。Objective：By observing cyclical pressures of different frequencies on rabbit chondrocyte proliferation and se -cretion of integrin α1β1, to study the effects and mechanism on rabbit chondrocytes .Method：Isolated fetal rabbit articular chondrocytes , the third-generation cells were seeded in plates .Set control group A （ normal culture ） and group B of different frequencies pressure （pressure 172kPa, frequency group B1 0.2Hz, group B2 0.5Hz, group B3 1Hz, group B4 2Hz, group B5 5Hz） .Pressure intervention of different frequencies 2 hours per day , 3 consecutive days after the intervention , to detect the proliferation of chondrocytes by Optical Density （ OD） , and ELISA assay was used to measure the integrin α1β1 level of culture supernatant.Result：OD value of group A was 1.08 ＆#177;0.13, group B1 was 1.19 ＆#177;0.11, group B2 was 1.23 ＆#177;0.08, group B3 was 1.35 ＆#177;0.06, group B4 was 1.45 ＆#177;0.09, group B5 was 1.37 ＆#177;0.12.Pressure groups compared with the control group was increased, the difference was statistically significant .There is significant difference between groups B , the peak appeared in B4 group.Group A of integrin α1β1 level was 3.02 ＆#177;0.26 pg/mL, group B1 was 3.28 ＆#177;0.19 pg/mL, group B2 was 3.43 ＆#177;0.11 pg/mL, group B3 was 3.77 ＆#177;0.24pg/mL, group B4 was 4.01 ＆#177;0.09 pg/mL, group B5 was 3.94 ＆#177;0.13 pg/mL.Integrinα1β1 value of pressure groups compared with the control group was increased , the difference was statistically significant （ P＆lt;0. 05） .There was significant difference between groups B; the peak appeared in group B 4.Conclusion： The same size （ 172 kPa） cyclic pressure stimuli on rabbit chondrocytes at different frequencies can promote chondrocyte proliferation and increase the integrins , but not the higher the frequency , the stronger the effect.In this study, the frequency of 2Hz has the strongest effect.

关 键 词：骨关节炎 软骨细胞 周期性压力 细胞增值 整合素α1β1 

分 类 号：R684.3[医药卫生—骨科学]