肿瘤负荷对胃癌患者免疫功能的影响  

Influence of tumor load on the immune function of patients with gastric cancer

作　　者：王婷婷[1] 温雯静[1] 岳麓旭[2] 

机构地区：[1]漯河医学高等专科学校病原生物学与免疫学教研室,462000 [2]漯河医学高等专科学校医疗系临床教研室,462000

出　　处：《国际医药卫生导报》2014年第19期2946-2949,共4页International Medicine and Health Guidance News

摘　　要：目的探讨胃癌患者外周血T淋巴细胞、单核细胞的表面分子变化及Th1／Th2的漂移，并分析肿瘤负荷对胃癌患者的免疫功能的影响。方法选取胃癌患者30例及同期体检的健康志愿者30例，采用流式细胞仪检测和分析外周血T淋巴细胞、单核细胞的表面分子及Th1／Th2细胞因子的变化。结果T淋巴细胞中CD3^＋、CD4^＋28^＋／CD3^＋、CD8^＋28^＋／CD3^＋水平均低于对照组（P〈0．01）；CD8^＋3^＋，CD28^-较正常组升高（P〈0．01）；单核细胞的表面分子CD40与正常对照组相比差异无统计学意义（P〉0．05），但是CD80与CD86均相对于正常水平降低（P〈0．01）；Th1细胞因子（IL-2、IFN-γ）降低（P〈0．01）；Th2细胞因子（IL-4、IL-10）的表达较正常组升高（P〈0．01）。结论胃癌患者的免疫功能受损，胃癌的发病可能与免疫功能低下有一定关系；Th1／Th2失衡，表明肿瘤细胞出现免疫逃避与胃癌的发展可能也有关系。Objective To investigate the changes of surface molecules in T lymphocyte, mononuelear and the shift of Th1/Th2 in patients with gastric cancer and to analyze the influence of the tumor load on the immunity function with gastric cancer. Methods Peripheral blood samples were collected from 30 preoperative patients with gastric cancer and 30 healthy controls. The changes of surface molecules in T lymphocyte, mononuclear and the state of Th1/Th2 were investigated and analyzed by flow eytometry. Results The levels of CD3^＋, CD4^＋28^＋/CD3^＋, CD8^＋28^＋/CD3^＋ were lower than those in control group （P 〈 0.01）; CD8^＋3^＋/ CD28^- was higher than that of the normal group （P 〈 0.01）; the mononuelear surface molecules CO40 was not significantly different with that of normal group （P 〉 0.05）, but the CD80 and CD86 were all lower than those of the normal （P 〈 0.01）; Thl eytokines （IL-2, IFN- γ） were all lower （P 〈 0.01）; Th2 eytokines （IL-4, IL-10） were all higher than those in the normal （P 〈 0.01）. Conclusion The cellular immunity of patients with gastric cancer was inhibited, which maybe related to the occurrence of gastric carcinoma; Th1/Th2 was out of balance, the result indicated the immune escape of tumor cell maybe also relevant with the development of gastric cancer.

关 键 词：肿瘤负荷 胃癌 TH1/TH2 

分 类 号：R735.2[医药卫生—肿瘤]