香港海鸥型菌对喹诺酮耐药性的检测分析  

Analysis on testing of quinolone resistance in Laribacter Hongkongensis

作　　者：朱丽东[1] 肖锋[1] 杨羚[2] 陈定强[2] 

机构地区：[1]广州医科大学金域检验学院,510180 [2]广州医科大学附属第一医院检验科,510120

出　　处：《实用医学杂志》2014年第19期3166-3169,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81201341)

摘　　要：目的:研究香港海鸥型菌对喹诺酮药物的耐药性情况,并评估琼脂稀释法和纸片扩散法对药敏结果的影响。方法:采用琼脂稀释法和纸片扩散法,对不同来源香港海鸥型菌分离株进行4种喹诺酮类抗生素药敏实验,并对两种检测方法所得结果进行比较。结果:136株香港海鸥型菌对4种常见喹诺酮类抗生素敏感率依次为左氧氟沙星>环丙沙星>诺氟沙星>萘啶酮酸。鱼分离株对左氧氟沙星均敏感,对诺氟沙星和环丙沙星的敏感率达到90%以上。蛙分离株对左氧氟沙星、诺氟沙星、环丙沙星和萘啶酮酸的敏感率分别为62.50%、37.50%、48.21%、19.64%。采用两种方法检测鱼分离株对左氧氟沙星和环丙沙星两种药物的药敏试验结果具有较好的一致性(>95%),对诺氟沙星具有完全一致性(100%)。对于蛙分离株,两种方法的一致性则较差。结论:香港海鸥型菌对喹诺酮类抗生素的耐药率较高,在蛙分离株中尤为明显。两种方法测定喹诺酮耐药性结果有一定差异,建议使用稀释法进行药敏测试。Objectives To investigate the prevalance of quinolone resistance in Laribacter hongkongensis and to evaluate the influence of agar dilution and disc diffusion methods on susceptibility testing for the bacterium. Methods Susceptibility to quinolones of L.hongkongensis was tested by agar dilution and disc diffusion methods. The results for both methods were compared. Results L.hongkongensis isolates exhibited different susceptibility rate for quinolones （levnfloxacin 〉 ciprofloxacin 〉 norfloxacin 〉 nalidixic acid）. All of the fish isolates were susceptible to levofloxacin and more than 90% were susceptible to ciprofloxacin and Norfloxacin. However,by agar dilution, only 62.50%, 37.50%,48.21%, 19.64% of the frogs isolates were susceptible to levofloxacin, norfloxacin, ciprofloxacin, nalidixic acid, respectively. For fish isolates, comparision of susceptibilities between agar dilution and disc diffusion showed a high （〉 95%） percentage agreement for levofloxacin and ciprofloxacin and the highest crepancies were observed with norfloxacin （100%） .But it showed a high discrepancy when comparing the two methods for frogs isolates. Conclusions The resistance rate of L. hongkongens to quinolones was high, especially for frog isolates. The results suggested the use of agar dilution on susceptibility testing for quinolones in L. hongkongensis.

关 键 词：香港海鸥型菌 喹诺酮类药物 敏感性 

分 类 号：R446.5[医药卫生—诊断学]