用DNA条形码技术鉴定阿拉善黄鼠和达乌尔黄鼠  

Identification of Spermophilus alaschanicus and Spermophilus dauricus by DNA barcoding

作　　者：陈宝宝[1] 孙养信[1] 安翠红[1] 霍丽霞[1] 范锁平[1] 

机构地区：[1]陕西省疾病预防控制中心鼠布生防科,西安710054

出　　处：《中国媒介生物学及控制杂志》2014年第5期432-434,共3页Chinese Journal of Vector Biology and Control

基　　金：陕西省科学技术研究发展计划项目(2012K16-12-03)~~

摘　　要：目的 探索阿拉善黄鼠和达乌尔黄鼠的分类鉴定方法。方法 运用DNA条形码技术测定两地黄鼠的线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）基因序列,分析遗传距离,构建系统发育NJ树。结果 11份阿拉善黄鼠的种内遗传距离〈2%,4份达乌尔黄鼠的种内遗传距离〈1%,两地黄鼠的遗传距离介于8%~9%之间,同时,NJ树将两地黄鼠分成2个独立分支。结论 阿拉善黄鼠为独立物种。DNA条形码技术可用于黄鼠种类鉴定。Objective To investigate the classification and identification methods for Spermophilus alaschanicus and S. dauricus. Methods DNA barcoding was used to determine the cytochrome c oxidase subunit Ⅰ （CO Ⅰ ） gene sequences of S. alaschanicus and S. dauricus, analyze the genetic distance, and construct a neighbor-joining （NJ） tree. Results The intraspecific genetic distance of S. alaschanicus （n= 11） was less than 2%, and that of S. dauricus （n=4） was less than 1%. The genetic distance between S. alaschanicus and S. dauricus ranged from 8% to 9%. Results of NJ tree showed that all the samples of the two species formed two independent groups with a high support value. Conclusion Spermophilus alaschanicus is an independent species. DNA barcoding can be used in the species identification of Spermophilus.

关 键 词：DNA条形码 细胞色素C氧化酶亚基Ⅰ基因 鼠种鉴定 阿拉善黄鼠 达乌尔黄鼠 

分 类 号：S443[农业科学—农业昆虫与害虫防治]