高比表面介孔碳的制备及其对溶菌酶的吸附研究  

Synthesis of Mesoporous Carbon with High Surface Area and Its Adsorption Property to Lysozyme

作　　者：孙何[1] 李群艳[1] 韦奇[1] 聂祚仁[1] 

机构地区：[1]北京工业大学材料科学与工程学院,北京100124

出　　处：《人工晶体学报》2014年第8期2142-2148,共7页Journal of Synthetic Crystals

基　　金：国家高技术研究发展计划(863计划)(2009AA03Z213);国家自然科学基金(21171014;50502002)

摘　　要：以间苯二酚-甲醛为碳源前驱体,正硅酸乙酯为硅源前驱体,嵌段聚合物F127(PEO106PPO70PEO106)为模板剂,在酸性条件下采用三组分共组装制备了具有介孔结构的碳硅复合材料。通过NaOH清洗去除硅元素可以得到具有更高面积和孔容的介孔碳。TEM和小角XRD结果表明,添加了正硅酸乙酯后所得介孔材料由有序结构变为无序蠕虫状。介孔碳材料的比表面积由389 m2/g增加到602 m2/g,孔容由0.45 cm3/g增加到0.58 cm3/g。所得介孔材料对溶菌酶的吸附量随着介孔材料比表面积和孔容的增加而增大。纯介孔碳的吸附行为符合Langmuir等温线吸附拟合,高比表面介孔碳的吸附过程符合Freundlich等温线吸附拟合。Mesoporous carbon-silica nanocomposites have been successfully synthesized by one-pot coassembly method,using resorcinol and formaldehyde as carbon precursor,tetraethoxysilane as silica precursor and triblock copolymer F127 as template. Mesoporous carbon with higher BET surface area and pore volume could be obtained by removing the silica with NaOH. The characterization results of TEM and SAXRD revealed that mesostrucetre of the mesoporous materials changed from ordered channel to disordered worm-like structures before and after the additive of TEOS. The BET surface area of the obtained materials increased from 389 m2/g to 602 m2/g,and pore volumes increased from 0. 45 cm3/g to 0. 58 cm3/g. The pore size of the mesoporous composites increased from 7. 48 nm to 7. 73 nm slightly.The immobilization amount of lysozyme adsorbed on mesoporous composties increased with the increase of the BET surface area and pore volume of mesoporus materials. The immobilization results show that the adsorption process of the pure mesoporous carbon follow the Langmuir isotherm and the materials with higher surface area and pore volume follow the Freundlich isotherm.

关 键 词：介孔碳 一步法共组装 酶固定 吸附等温线 

分 类 号：TQ424.1[化学工程]