瑞舒伐他汀联合氯沙坦对ox-LDL诱导的人单核巨噬细胞小凹蛋白-1表达的影响  

Effects of Rosuvastatin and Losartan on expression of caveolin-1 in cultured human monocyte-macrophage cells induced by oxidized low density lipoprotein

作　　者：贺彧彬 柳丽娜[1] 卫政[1] 吉鹏娟 申晓彧[1] 

机构地区：[1]山西医科大学第二医院心血管内科,太原030001

出　　处：《中国医师杂志》2014年第8期1052-1055,共4页Journal of Chinese Physician

基　　金：山西省科技攻关项目（20120313020-10）

摘　　要：目的 探讨瑞舒伐他汀、氯沙坦以及二者联合对氧化修饰低密度脂蛋白（ox-LDL）诱导的人单核巨噬细胞小凹蛋白-1表达的影响.方法 分离培养人单核细胞并诱导转化为巨噬细胞,建立动脉粥样硬化模型,分别以不同剂量瑞舒伐他汀（0.1、1.0、5.0 μmol/L）、氯沙坦（10、50、100μmol/L）以及联合两药最适剂量（5.0 μmol/L＋ 100 μmol/L）干预细胞,以RT-PCR检测各组小凹蛋白-1的mRNA表达.结果 与空白对照组比较,ox-LDL组小凹蛋白-1 mRNA表达显著减少[（0.2533±0.00973） vs （0.9410 ±0.03677）],其差异有统计学意义（P＜0.01）;与ox-LDL组比较,随着瑞舒伐他汀及氯沙坦浓度的升高,小凹蛋白-1 mRNA表达均逐渐升高[（0.5198±0.04840）、（0.6183±0.06740）、（0.7257±0.03052） vs（0.2533±0.00973）;（0.3350 ±0.04177）、（0.4428 ±0.03804）、（0.6049 ±0.02627） vs （0.2533 ±0.00973）],各组间比较差异有统计学意义（P＜0.05）;最适剂量瑞舒伐他汀及氯沙坦联合组与单药组比较,小凹蛋白-1 mRNA表达显著升高,各组间比较差异均有统计学意义（F =59.119,P＜0.01）.结论 瑞舒伐他汀及氯沙坦均可以通过ox-LDL途径上调人单核巨噬细胞小凹蛋白-1 mRNA表达,且呈一定的剂量依赖趋势;两药以最适剂量联合应用后,小凹蛋白-1 mRNA表达较单药更为显著.Objective To investigate the effects,mechanisms,and the optimum doses of Rosuvastatin and Losartan on expression of caveolin-1 in cultured human monocyte-macrophage cells which were induced by oxidized low density lipoprotein（ox-LDL）.Methods Human-monocyte cells were separated and changed into the human monocyte-macrophage cells.The model of amerosclerosis was set up.These cells were incubated in different doses of Rosuvastatin（0.1,1.0,5.0 μmol/L） and Losartan （10,50,100 μmol/L）,and then cultured in combination of two drags （5.0 μmol/L ＋ 100 μmol/L）.Expression of caveolin-1 mRNA was determined with real-time fluorescent quantitative polymerase chain reaction （RT-PCR）.Results In ox-LDL group,caveolin-1 mRNA was decreased sharply relative to control group [（0.2533 ±0.00973） vs （0.9410 ±0.03677）] in a concentration-dependent manner （P ＜0.01）.Compared to ox-LDL group,expressions of Caveolin-1 mRNA were increased gradually in different doses of Rosuvastatin alone and Losartan alone group [（0.5198 ± 0.04840）,（0.6183 ± 0.06740）,（0.7257 ± 0.03052） vs （0.2533 ± 0.00973） ; （0.3350 ± 0.04177）,（0.4428 ± 0.03804）,（0.6049 ± 0.02627） vs （0.2533 ± 0.00973）] in a concentration-dependent manner （P ＜ 0.01） ; the summit expressions of caveolin-1 mRNA were emerged in using Rosuvastatin and Losartan together （F =59.119,P ＜ 0.01）.Conclusions Rosuvastatin and Losartan may be responsible for the expression of caveolin-1 in human monocyte-macrophage cells that were induced by ox-LDL.The expressions were up-regulated with dose dependent manner of these drugs,and got the crest stage when using optimum doses of Rosuvastatin and Losartan together.

关 键 词：降血脂药/药理学 氟苯类/药理学 嘧啶类/药理学 磺胺类/药理学 联苯化合物/药理学 四唑类/药理学 咪唑类/药理学 脂蛋白类 LDL 单核巨噬细胞系统/药物作用 细胞质膜微囊蛋白/药物作用 

分 类 号：R96[医药卫生—药理学]