宫颈上皮内瘤变患者淋巴细胞亚群的分析  

Analysis of T lymphocyte subsets in peripheral blood of patients with cervical intraepithelial neoplasia

作　　者：吕江涛[1] 孙婷婷[1] 陈飞[1] 谭先杰[1] 

机构地区：[1]中国医学科学院,北京协和医学院,北京协和医院妇产科,北京100730

出　　处：《生殖医学杂志》2014年第9期743-747,共5页Journal of Reproductive Medicine

基　　金：国家自然科学基金项目(81172483)

摘　　要：目的了解宫颈上皮内瘤变(CIN)患者全身细胞免疫功能的变化。方法用特异性荧光标记素,通过流式细胞术对38例CIN患者及20例健康对照组的外周血中T淋巴细胞亚群进行检测。结果随着病变级别加重,CIN患者的CD4+T细胞比例逐渐下降,而CD8+T细胞比例逐渐增加,但差异无统计学意义。与健康对照相比,CIN2患者和CIN3患者的CD4+T细胞/CD8+T细胞的比值下降,分别是(1.46±0.43)、(1.05±0.52)和(0.99±0.58),差异有统计学意义。CD8+T细胞的激活亚群,CD4+T细胞和CD8+T细胞的纯真亚群、记忆亚群在各组之间没有明显的统计学差异。CIN3患者的CD4+T细胞中的激活亚群[(17.8±6.1)%]与健康对照组[(11.5±5.4)%]有显著性差异。结论 CIN患者的全身细胞免疫功能出现异常,为免疫治疗干预癌前病变发展提供了理论基础。Objective：To evaluate the characteristics of systemic cell-mediated immunity in patients with cervical intraepithelial neoplasia（CIN）.Methods：Specific fluorescent markers were used to detect T lymphocyte subsets in peripheral blood of 38 CIN patients and 20 normal controls by flow cytometry.Results：With the increased level of lesions,the proportion of CD4＋T cells of patients with CIN decreased,while that of CD8＋T cells gradually increased,but the results were not statistically significant.Compared with healthy controls,the ratio of CD4＋T cells to CD8＋T cells in patients with CIN 2（1.05±0.52）and CIN 3（0.99±0.58）were significantly decreased compared with normal controls（1.46±0.43）（P〈0.05）.No significant difference was detected in activated subsets of CD8^＋T cells,naive subsets and memory subsets of CD4^＋T cells and CD8＋T cells among the groups.Activated subsets of CD4^＋T cell in patients with CIN3 were more than that in healthy controls[（17.8±6.1）%vs.（11.5±5.4）%].Conclusions：The systematic immunity in patients with CIN has been altered by disease,which lays a foundation of immunological therapy for these patients.

关 键 词：宫颈上皮内瘤变 人乳头瘤病毒 淋巴细胞亚群 

分 类 号：Q492.5[生物学—生理学]