香烟烟雾提取物对小鼠精子活力的影响  

Effects of cigarette smoke condensate on sperm motility in mouse

作　　者：余波澜[1] 蒋露[1] 郑婷[1] 周华[1] 孙筱放[1] 

机构地区：[1]广东省普通高校生殖与遗传重点实验室,广州医科大学附属第三医院,广州市510150

出　　处：《生殖医学杂志》2014年第9期754-759,共6页Journal of Reproductive Medicine

基　　金：国家自然科学基金资助(81370751)

摘　　要：目的探讨香烟烟雾中所含外源活性氧物质(ROS)对小鼠精子活力的影响。方法香烟燃点后烟雾溶于二甲亚砜,配制成高ROS含量的香烟烟雾浓缩物(CSC)。釆用4周龄C57BL/6J小鼠45只,实验设置对照组(0mg/ml CSC)、低剂量组(0.2mg/ml CSC)和高剂量组(2.0mg/ml CSC)。连续饲喂含有CSC的饮用水4周,观察小鼠的生殖系统发育情况和可能的全身毒性反应,并检测肝功能指标。处死小鼠后即刻检测精子浓度和活力,检测睾丸内抗氧化酶类的表达水平,并制片观察睾丸组织病理变化。结果与对照组相比,低剂量和高剂量组小鼠精子浓度无显著变化,但高剂量组精子活力呈现显著下降。CSC低剂量组和高剂量组小鼠连续饲喂4周后精子总活动力分别为32%和19%,A级活动精子分别为13%和12%,均显著低于对照组(61%和24%)(P<0.05)。但在本实验剂量范围内,CSC对小鼠发育和生殖系统无明显毒性损伤,对睾丸抗氧化基因表达无显著影响。结论 CSC对小鼠精子活力有显著影响,但是对小鼠全身和生殖系统无明显毒性作用。CSC饲喂法可推荐用于诱导小鼠弱精子症模型。Objective：To study the effect of exogenous reactive oxygen species（ROS）contained in cigarette smoke condensate（CSC）on sperm motility in mouse.Methods：Cigarette smoke was dissolved into DMSO to make the cigarette smoke condensate（CSC）,which contains high concentration of ROS.Forty five C57BL/6Jmice of 4-week old were randomly divided into three groups：control group,low dose group（0.2mg/ml CSC）,and high dose group（2.0mg/ml CSC）.The three groups were fed with water with or without different concentration of CSC for 4weeks.After the mice were sacrificed,the CSC toxicity and development of reproductive system were evaluated.In addition,the liver function and sperm morphology,concentration and motility were analyzed.Furthermore,the expression of antioxidant enzymes and pathologic alterations in the testis were examined.Results：There was no significant difference in sperm concentration among the three groups.The sperm motility was significantly decreased in the two treatment groups（19% in the high dose group,32% in low dose group）compared with the controls（61%）（P〈0.05）.The percentage of sperm with rapid progressive motility was 12%,13%,and 24% in the three groups respectively.CSC did not cause toxicity on body development and reproductive system.CSC also did not affect the liver function or the antioxidant gene expression in testis.Conclusions：CSC treatment significantly affects sperm motility,without obvious toxicity on reproductive system,liver function,and body development in mice.This method might be used to establish the asthenozoospermic mouse model.

关 键 词：香烟烟雾提取物 精子活力 活性氧物质 弱精子症 小鼠模型 

分 类 号：Q492.4[生物学—生理学]