异位预成中心性血管化人工骨的构建  

Construction of the central vascularized bone

作　　者：王思明[1] 阚娜[1] 张磊[2] 

机构地区：[1]连云港市第二人民医院口腔科,江苏省连云港市222023 [2]连云港市第二人民医院病理科,江苏省连云港市222023

出　　处：《中国组织工程研究》2014年第33期5271-5275,共5页Chinese Journal of Tissue Engineering Research

基　　金：连云港市卫生局科研基金资助项目(1223)~~

摘　　要：背景:血管化在骨形成和改建中起重要作用,生物活性陶瓷β-磷酸三钙的多孔性及可吸收性为血管植入构建血管化人工骨提供可能。目的:探讨异位预成中心性血管化人工骨的建立、血供及人工骨血管化的机制,为异位预成中心性血管化人工骨的临床应用提供理论依据。方法:选择新西兰兔的腰背动脉解剖分离形成血管束,实验侧将血管束移入人工骨侧槽内,并用自体微小骨颗粒填满,埋入背阔肌肌袋内,以未做血管束植入动物作为对照。术后4,6,8周行大体形态学和组织学检查。结果与结论:血管束植入组人工骨形成类似滋养孔的结构,血管丰富,血管通畅。中心性血管化人工骨各处均有大量血管新生,4-8周充满全层,4周时开始出现新生骨和骨代谢,12周新生骨更趋于成熟。对照组仅少量血管自周围长入,新生骨少且不成熟。结果表明中心性血管化人工骨可显著促进人工骨血管化。该模型有望成为磷酸钙人工骨的临床应用的新方式。BACKGROUND：The vascularization plays a key role in bone formation and reconstruction. Bioactiveβ-tricalcium phosphate has a porosity and absorbability that al ow a possibility of constructing vascularized bone. OBJECTIVE：To explore the blood supply and construction of the central vascularizedβ-tricalcium phosphate, as wel as the mechanism of vascularization in artificial bone for its further clinical application. METHODS：The lumbar dorsal artery of New Zealand rabbits was isolated and prepared into vascular bundle. At the experimental side, the vascular bundle was implanted into channel of theβ-tricalcium phosphate, then fil ed with the autologous tiny bone particles, and embedded into the latissimus dorsi muscle. Animals without the vascular bundle implantation were taken as the control group. The samples were determined for the morphology and histology at 4, 8, and 12 weeks post-surgery. RESULTS AND CONCLUSION：The site where the vascular bundle was implanted appeared foramen nutriens-like structure, the blood vessels were abundant and smooth. The central vascularized tricalcium phosphate were fil ed with new-born vessels at 4-8 weeks. The newly formed bone and bone metabolism appeared at 4 weeks and newly formed bones were more mature at 12 weeks. In the control group, there were only a few new-born vessels, the newly formed bone was immature. The central vascularizedβ-tricalcium phosphate can significantly improve the vascularization of artificial bone. This model can probably serve as a new method for tricalcium phosphate in clinical use.

关 键 词：组织构建 骨组织工程 异位骨形成 中心性血管化 预成骨 人工骨 

分 类 号：R318[医药卫生—生物医学工程]