以脂肪源性干细胞膜片构建组织工程皮肤  

Tissue-engineered skin constructed by adipose-derived stem cell sheet

作　　者：肖斌[1] 刘毅[1] 

机构地区：[1]解放军兰州军区兰州总医院全军烧伤整形外科中心,甘肃省兰州市730050

出　　处：《中国组织工程研究》2014年第33期5361-5365,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:细胞膜片技术以细胞自分泌的透明质酸及胶原蛋白做为内源性支架,能消除外源性支架的各种弊端。人脂肪源性干细胞结合细胞膜片技术用于全层皮肤缺损成为当前的研究热点。目的:综述脂肪源性干细胞膜片研究现状及进展。方法:计算机检索维普、PubMed数据库中1993年1月至2014年4月关于脂肪源性干细胞、细胞膜片技术及皮肤组织工程相关文章。在关键词中以"脂肪源性干细胞,创面愈合,组织工程皮肤,细胞膜片"或"adipose-derived stem cell,wound healing,tissue-engineered skin,cell sheet"为检索词进行检索。最终选择27篇文献进行分析。结果与结论:来源于人废弃脂肪组织的脂肪干细胞具有成体干细胞的多向分化潜能和免疫相容性,可理想的用于再生医学。细胞膜片技术与传统的外源性可降解材料支架构建的组织相比具有多种优势。脂肪源性干细胞结合细胞膜片技术相结合,有利于组织血管化,能从多个方面促进创面愈合,其所分泌的细胞外基质层能提供适宜于细胞分化和生长的微环境,对全层皮肤缺损创面愈合具有正调控作用,可望构建真正的3-D化组织工程皮肤。BACKGROUND：Wtih hyaluronic acid and col agen by cellautocrine as endogenous scaffolds, cellsheet technology can eliminate the disadvantages of exogenous scaffolds. Human adipose-derived stem cells combined with cellsheet technology used in the study of ful-thickness skin defects have become the current hot spot. OBJECTIVE：To summarize the research progress in adipose-derived stem cellsheet. METHODS：Articles related to adipose-derived stem cells, cellsheet technology and tissue-engineered skin were retrieved in VIP information and PubMed database from January 1993 to April 2014. The key words were“adipose-derived stem cell, wound healing, tissue-engineered skin, cellsheet”in English and Chinese, respectively. Total y 27 relevant articles were analyzed. RESULTS AND CONCLUSION：Adipose-derived stem cells derived from discarded human adipose tissue are immmunocompatible and multi-potent, rendering them ideal for regenerative medicine applications. cellsheet technology seemingly provides several advantages over traditional tissue reconstruction with biodegradeable scaffolds. Adipose-derived stem cells combined with cellsheet technology can enhance tissue vascularization, promote wound healing, the secreted extracellular matrix provides the microenvironment for the celldifferentiation and growth, so it is expected to build a real three-dimensional tissue-engineered skin.

关 键 词：组织构建 组织工程 脂肪源性干细胞 创面愈合 皮肤缺损 细胞膜片 血管化 透明质酸 胶原蛋白 

分 类 号：R318[医药卫生—生物医学工程]