一次性静脉注射高剂量链脲佐菌素建立1型糖尿病小型猪模型  

Establishment of a miniature pig model of type 1 diabetes by single intravenous injection of high dose streptozotocin

作　　者：崔永春[1] 李凯[1] 张宏[1] 刘晓鹏[1] 袁卫民[1] 李巨波[1] 吴爱丽[1] 唐跃[1] 

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心,阜外心血管病医院动物实验中心,心血管疾病国家重点实验室,北京市心血管植入材料临床前评价重点实验室,北京100037

出　　处：《中国实验动物学报》2014年第4期12-15,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京市科委科技计划"常见心脑血管缺血性疾病模型研发公共平台建设"(Z101107052210004)基金;协和青年基金"糖尿病小型猪急性心肌缺血代谢组学研究(2012-XHQN04)支持

摘　　要：目的：探讨通过一次性注射高剂量链脲佐菌素（ streptozotocin，STZ）方法建立1型糖尿病小型猪模型的可行性。方法中华实验小型猪耳缘静脉一次性注射链脲佐菌素溶液150 mg/kg，分别在给药前和给药后10 min、30 min、90 min、第1天、第2天、第3天和第7天空腹采集静脉血，动态监测空腹血糖，并利用静脉糖耐量实验和C肽释放实验对模型进行鉴定。结果给药后第1天开始，模型组空腹血糖明显升高并始终维持在16.7～20.6 mmol/L的浓度范围，达到糖尿病标准；静脉葡萄糖耐量试验和C肽释放实验结果表明，静脉注射体积分数50％的葡萄糖1 h后模型猪血糖浓度高于11.1 mmol/L，2 h后未能恢复至空腹血糖水平；而胰岛素和C肽在注入葡萄糖后基本未发生任何反应，始终保持痕量水平。结论一次性静脉注射大剂量链脲佐菌素的方法能够成功建立1型糖尿病小型猪模型。Objective To explore the feasibility of establishing mini-pig model of type 1 diabetes by single intrave-nous injection of high dose streptozotocin .Methods Eight male Zhonghua mini-pigs （22.6 ＆#177;1.8 kg） were administrated with high-dose streptozotocin （150 mg/kg） into the ear vein .Before and 10, 30, 90 minutes, 1, 3 and 7 days after adminis-tration of streptozotocin , blood samples were obtained respectively , and used to dynamically monitor the fasting blood-glucose. C peptide and insulin levels were evaluated by IVGTT test .Results Since 24 hours after STZ administration , the fasting blood-glucose level was increased significantly compared with that of pre-administration and maintained at 16.7-20.6 mmol/L, while the C peptide and insulin levels were decreased significantly .IVGTT results showed that blood sugar levels at 1 h af-ter intravenous injection of 50%glucose were much higher than 11.1 mmol/L and failed to restore to fasting glucose levels until 2 h, insulin and c-peptide did no response after injection of glucose , always kept at a trace level .Conclusion A sin-gle high-dose streptozotocin injection can be used to establish a mini-pig model of type 1 diabetes successfully .

关 键 词：1型糖尿病 小型猪 链脲佐菌素 血糖 

分 类 号：Q95-33[生物学—动物学]