移植部位对小鼠睾丸移植效果的影响  

Effect of different transplantation sites on the outcome of testicular grafts in mice

作　　者：张艺宝[1] 李淼[2] 郁丽丽 强苏静 刘丽均 徐平[2,3] 芮荣[1] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]中国科学院上海生命科学研究院,上海201615 [3]上海斯莱克实验动物有限责任公司,上海201615

出　　处：《中国实验动物学报》2014年第4期56-59,I0007,I0008,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划课题(2013BAK11B02);上海市科学技术委员会科研计划项目(11140902700)

摘　　要：目的比较不同的移植部位对睾丸移植效果的影响,以探索建立疾病模型小鼠安全保存的新方法。方法按移植部位的不同分为3组:背部皮下组,睾丸白膜内组和肾包膜下组。移植后处死受体鼠回收移植物。测试和分析受体精囊的重量,移植物存活率,回收物增重的倍数和生殖细胞的分化情况。结果不同实验组移植睾丸的生殖细胞分化差异显著。睾丸白膜内组的分化情况最好与假移植对照组相似;背部皮下移植组睾丸的分化率为29.2%;肾包膜下组未见分化。结论睾丸白膜内移植最利于精子的发生;背部皮下移植是睾丸移植的次优选择;肾包膜下睾丸移植不适合用于小鼠雄性生殖器官的安全保存。Objective To compare the effects of different transplantation sites on the outcome of testicular grafts in mice, and to provide a basis for development and application of this technique in relevant research .Method 5-day old and 4-week old SPF male C57BL/6J mice were used in this study .Three groups of testicular transplantation , i.e.dorsal subcutaneous transplantation （ 5 mice, 40 testes ） , transplantation inside the testicular tunica albuginea （ 6 mice, 12 testes）, and were subrenal capsule transplantation （10 mice,15 testes） groups were set up for evaluating the effect of transplantation site on the outcomes in mice .Sham operation （4 mice） and castration （4 mice） groups were also used in this study.The mice were sacrificed at 8 weeks after transplantation and the transplanted testes were collected for analysis of their weight, transplant survival, weight gain, and germ cell differentiation.Results There were significant differences of the testicular germ cell differentiation in different transplantation groups .The germ cell differentiation was best in the in-tra-tunica albuginea transplantation group , and were similar to that in the sham operation group .The germ cell differentia-tion rate was 100%in the intra-tunica albuginea transplantation group , 29.2% in the subcutaneous transplantation group and 0%in the subrenal capsule transplantation group .Conclusions Transplantation beneath the testicular tunica albug-inea is the most favorable site for germ cell differentiation , and dorsal subcutaneous transplantation is an alternative choice . Subrenal capsule transplantation is not appropriate for preservation of male reproductive organs in mice .

关 键 词：小鼠 睾丸移植 移植部位 生殖细胞分化 

分 类 号：S865.49[农业科学—野生动物驯养]