旋毛虫感染黑线仓鼠及其白化突变系后细胞因子变化比较分析  

Changes of cytokines in the Cricetulus barabensis and their albino mutant infected with trichinella spiralis

作　　者：张小飞[1] 刘冉[1,2] 崔晓霞[1] 马帅[1] 张西臣[3] 白杰英[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]北京城市学院生物医药学部,北京100081 [3]吉林大学畜牧兽医学院,长春130062

出　　处：《中国比较医学杂志》2014年第8期11-15,23,共6页Chinese Journal of Comparative Medicine

基　　金：军事医学科学院创新基金(2012CXJJ022);动物中心青年基金(QN2011005);"十二五"重大项目(2011zxj09201-031)

摘　　要：目的比较旋毛虫感染黑线仓鼠及其白化突变系后两种动物免疫状态的变化,建立适合感染寄生虫的动物模型。方法用旋毛虫感染黑线仓鼠及其白化突变系后,通过检测血细胞生理值、脾脏IL-2蛋白浓度和脾脏IL-6基因表达水平,分析两种动物在感染旋毛虫前后免疫应答水平及免疫调控状态的变化。结果黑线仓鼠在感染旋毛虫后免疫细胞及细胞因子的水平高于白化突变系,反应程度更加剧烈。结论黑线仓鼠是旋毛虫感染的较为适宜的模型动物。Objective To explore the differences in immune responses between Cricetulus barabensis and their albino mutant infected with Trichinella spiralis.Methods The physiological parameters of blood , expression levels of IL-2 protein and IL-6 gene in the spleen were analyzed.Results The level of immune cells and cytokines of Cricetulus barabensis was higher than that in the albino mutant .Conclusions Cricetulus barabensis is a suitable model animal for research on long-term latent infection such as infection with Trichinella spiralis.

关 键 词：旋毛虫 黑线仓鼠 白化突变系 细胞因子 

分 类 号：R33[医药卫生—人体生理学]