硫化氢通过抑制氧化应激改善高糖诱导的内皮细胞衰老  

Hydrogen sulfide ameliorates high glucose-induced endothelial cell senescence by suppressing oxidative stress

作　　者：宋志明[1] 王敏[1] 刘勇[1] 郝宝顺[1] 牛海名[1] 刘定辉[1] 余舒杰[1] 周彬[1] 吴琳[1] 余显冠 凌叶盛 彭沛[1] 朱洁明[1] 陈璘[1] 钱孝贤[1,2] 

机构地区：[1]中山大学附属第三医院心血管内科,广东广州510630 [2]中西医结合研究所,广东广州510630

出　　处：《中国病理生理杂志》2014年第8期1345-1350,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81370447);广东省科技计划(No.2011B031800024;No.2011B031800155)

摘　　要：目的:探讨外源性硫化氢对高糖诱导的人脐静脉内皮细胞(HUVECs)衰老的作用及机制。方法:建立高糖(33 mmol/L葡萄糖)诱导的HUVECs早熟型衰老模型,观察硫化氢对衰老的作用及其相关的分子机制。结果:HUVECs经高糖处理后,细胞生长缓慢,衰老相关β半乳糖苷酶染色(SA-β-Gal)阳性细胞数目增加,血浆纤溶酶原激活物抑制剂1(PAI-1)表达明显增高,超氧化物歧化酶1(SOD1)显著减少,丙二醛(MDA)产量明显增多,核因子κB p65(NF-κB p65)活性增加;与模型组比较,100μmol/L和200μmol/L硫氢化钠(硫化氢供体)处理组的细胞数目明显增加,SA-β-Gal染色阳性细胞数目及PAI-1蛋白表达显著下降,SOD1表达明显增加,MDA产量明显减少,NF-κB p65活性降低。结论:硫化氢能通过抑制氧化应激反应和NF-κB p65活性而抵抗高糖诱导的HUVECs衰老。AIM： To explore the effect of hydrogen sulfide on the senescence of human umbilical vein endothe -lial cells （HUVECs） induced by high glucose.METHODS： Senescence model was established by treating HUVECs with33 mmol/L glucose for 48 h.The parameters were detected to demonstrate the effect of hydrogen sulfide on senescence andthe mechanism involved was also investigated .RESULTS： In the cells treated with high glucose, the proliferation was attenuatedwith a higher number of senescence -associated β-galactosidase （SA-β-Gal） positive cells, and plasminogen activatorinhibitor 1 （PAI-1） protein expression, malondialdehyde （MDA） production and NF-κB p65 activity were increasedsignificantly, but the expression of superoxide dismutase 1 （SOD1） was decreased.However, the cell number and SOD1expression were increased, and the number of SA-β-Gal positive cells, PAI-1 protein expression, MDA production and theactivity of NF-κB p65 were decreased after sodium hydrosulfide （100 and 200 μmol/L） treatment.CONCLUSION： Exogenoushydrogen sulfide prevents HUVECs against high glucose -induced senescence by suppressing oxidative stress and NF -κB p65 activity.

关 键 词：硫化氢 内皮细胞 氧化性应激 NF-κB P65 高糖 

分 类 号：R339.38[医药卫生—人体生理学]