种植骨髓间充质干细胞脑血管支架的性能评价  

Performance evaluation of a cerebrovascular stent coated with bone marrow mesenchymal stem cells

作　　者：唐瑛[1] 刘李娜[1] 王庆敏[1] 刘畅[2] 杨义力[2] 

机构地区：[1]解放军海军医学研究所,上海市200433 [2]长征医院转化医学中心,解放军第二军医大学,上海市200433

出　　处：《中国组织工程研究》2014年第34期5503-5508,共6页Chinese Journal of Tissue Engineering Research

基　　金：上海市卫生局基金(12K004)~~

摘　　要：背景:一般认为对支架表面进行修饰,尤其是种植细胞,可能会加速或导致其内皮化,避免支架内血栓形成而引起再狭窄的发生。目的:寻找骨髓间充质干细胞种植血管支架的最佳条件。方法:将1×106,1×107,1×108,1×109 L-1的第3代大鼠骨髓间充质干细胞分别接种至不锈钢血管支架制备细胞-支架复合物,接种48 h后电镜观察细胞形态变化。取SD大鼠160只,20只为正常对照组,其余140只制作缺血性脑卒中模型,造模8周后随机分为7组,分别植入不锈钢支架、高分子材料支架及不同浓度细胞-支架复合物,以不植入任何材料的为模型对照。植入后8周,蛋白免疫印迹技术检测植入支架上细胞的血管内皮生长因子表达,流式细胞仪检测各组大鼠血小板活化。结果与结论:种植的干细胞均能在不锈钢支架上贴壁生长,当细胞浓度为1×107 L-1时,上皮样细胞完整覆盖支架,细胞及细胞器形态正常。缺血性脑卒中模型实验中,1×107 L-1细胞-支架组细胞生长良好,在血管内环境诱导下具有良好的内皮化趋势,并且可显著抑制血小板活化。表明当细胞种植浓度为1×107 L-1时制备的血管支架通畅,具有良好的生物学效应。BACKGROUND：Generaly, the stent surface modification, especialy seeding cels, may accelerate or cause stent endothelium, and cause restenosis for prevention of in-stent thrombosis. OBJECTIVE： To develop the optimal conditions for vascular stents coated with bone marrow mesenchymal stem cels. METHODS： Cerebrovascular stent was co-cultured with passage 3 bone marrow mesenchymal stem cels from rats at 1×10^6, 1×10^7, 1×10^8, and 1×10^9/L. Cels on the stents were examined with transmission electron microscopy after 48 hours. A total of 160 male Sprague-Dawley rats were enroled, among which, 20 rats were as normal control group, and the remaining 140 were used for producing models of ischemic stroke that were randomly sub-divided into seven groups at 8 weeks after modeling： stainless steel stent implanted group, polymer stent group, and different concentrations of cellstent composite groups. After 8 weeks of implantation, the expression of vascular endothelial growth factor in these cels was examined by western blot assay. Rat platelet activation in different groups was determined by flow cytometry. RESULTS AND CONCLUSION：Implanted stem cels were able to grow adherently on the stainless steel stent wal. When the planting cellconcentration was 1×10^7 cels/L, the cels and organeles were morphologicaly normal and covered the stent surface wel. These coated cels also expressed vascular endothelial growth factor, suggesting that they functioned as endothelial cels, and they also significantly lowered platelet activation. When co-cultured with 1×10^7/L bone marrow mesenchymal stem cels, the stent was covered wel with endothelial-like cels and had significant lower platelet activationin vivo.

关 键 词：生物材料 材料相容性 不锈钢血管支架 血管支架 骨髓间充质干细胞 血小板 

分 类 号：R318[医药卫生—生物医学工程]