模拟失重条件下大鼠心室肌Beclin-1和LC3的表达变化  

Effects of Simulated Weightlessness on Expression of Beclin-1 and LC3 in the Ventricular Muscle of Rats

作　　者：赵疆东[1] 王永春[1] 李小涛[1,2] 陈荣贵[1] 孙喜庆[1] 

机构地区：[1]第四军医大学航空航天医学系航空航天生物动力学教研室,陕西西安710032 [2]西安电子科技大学体育部,陕西西安710071

出　　处：《航天医学与医学工程》2014年第4期291-294,共4页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助项目(81171872和81372130)

摘　　要：目的探讨模拟失重大鼠心室肌自噬相关基因Beclin-1和微管相关蛋白轻链3(LC3)的表达变化。方法雄性SD大鼠12只,随机分为对照组和模拟失重组。模拟失重4周后取心室肌组织,采用RT-PCR和Western Blot方法测定Beclin-1和LC3 mRNA及蛋白表达的变化。结果与对照组相比,模拟失重4周后大鼠心室肌Beclin-1和LC3 mRNA及蛋白表达均显著增强(P<0.05)。结论模拟失重可诱导大鼠心室肌自噬相关基因Beclin-1和LC3的高表达,提示模拟失重可能引起了心室肌自噬的激活。Objective To explore the expression of autophagy related gene Beclin-1 and LC3 in the ventricular muscle after simulated weightlessness. Methods A total of 12 male SD rats were randomly assigned to control and simulated weightlessness groups. The ventricular muscle tissues of rats subjected to simulated weightlessness were taken after 4 weeks of simulated weightlessness,respectively. RT-PCR and Western Blot were applied to determine the expression of autophagy related gene Beclin-1 and LC3. Results Compared with the control group,the expression of Beclin-1 and LC3 mRNA and protein in the ventricular muscle were both significantly increased after 4 weeks of simulated weightlessness. Conclusion Simulated weightlessness increases the expression of autophagy related gene Beclin-1 and LC3,suggesting autophagy may be induced by simulated weightlessness in the ventricular muscle of rats.

关 键 词：模拟失重 心室肌 自噬 基因表达 

分 类 号：R854[医药卫生—航空、航天与航海医学]