一株H5N2亚型禽流感病毒的分离鉴定及生物学特性研究  

Biological analysis and pathogenicity of an H5N2 avian influenza virus isolated from chicken

作　　者：慈彦鹏[1] 王德丽[1] 樊兆斌[1] 刘丽玲[1] 贾莹[1] 李鑫磊[1] 田国彬[1] 曾显营[1] 陈化兰[1] 李雁冰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/国家禽流感参考实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2014年第9期672-675,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：农业部公益性行业专项-边境地区动物疫病防控技术体系研究(201103008);农业部动物流感监测与防治专项

摘　　要：2013年12月在河北省保定市某鸡场分离到一株H5N2亚型禽流感病毒(AIV),命名为A/chicken/Hebei/3/2013(H5N2)。本研究对其全基因组序列进行测序,并对鸡进行静脉接种指数(IVPI)测定,同时对BALB/c小鼠进行致病性试验。病毒的各基因节段序列分析表明:该病毒的HA基因属于Clade 7.2分支,HA蛋白的裂解位点处具有多个连续碱性氨基酸(342RRRKR346),具备高致病性AIV(HPAIV)的典型分子特征。BLAST分析显示该分离株的各个基因节段分别与H5N1和H9N2亚型AIV的相应基因节段具有很高的同源性,推测该分离株可能为一株重组病毒。该病毒的IVPI为2.94,对鸡呈高致病性;对鼠的半数致死量(MLD50)>6.0 logEID50,呈低致病性,病毒仅在小鼠的肾和鼻甲骨中进行较低程度的复制。以上结果表明,Clade 7.2分支HPAIV仍保持对鸡高致病性而对小鼠低致病性的特征。An H5N2 subtype avian influenza virus （AIV） A/chicken/Hebei/3/2013 （H5N2） （CK/HeB/3/13） was isolated fi＇om the influenza outbroken chicken flocks in Baoding, Hebei province which was subjected to genome sequencing, intravenous pathogenicity index （IVPI） test and pathogenic study in BALB/c mice. The genome analysis of HA demonstrated that the virus was belonged to Clade 7.2 with a polybasic motif 342RRRKR346 at the HA cleavage site, which was the molecular characterization of highly pathogenic A1V （HPA1V）. The sequence alignment results revealed that the isolate was a natural reassortant AIV of H5N1 and H9N2 subtypes. Animal experiments indicated that the virus was highly pathogenic to chicken with IVPI of 2.94 and low pathogenic to mice with MLD50〉6.0 log EIDs0, indicating that the pathogenicity of the reassortant AIV isolate was still consistent with the Clade 7.2 HPAIV.

关 键 词：禽流感病毒 H5N2 重组 进化分析 致病性 

分 类 号：S852.65[农业科学—基础兽医学]