连云港地区规模猪场猪嵴病毒感染流行病学调查研究  

Epidemiological investigation of porcine kobuvirus in pigs of Lianyungang area

作　　者：单玉平[1] 王益军[1] 李锋[1] 张明敬[1] 邓建新[2] 张松武[3] 黄继康 徐运芳[5] 

机构地区：[1]连云港市畜牧兽医站,江苏连云港222000 [2]赣榆县畜牧兽医站,江苏赣榆222100 [3]东海县畜牧兽医站,江苏东海222300 [4]灌云县畜牧兽医站,江苏灌云222200 [5]灌南县畜牧兽医站,江苏灌南222500

出　　处：《中国预防兽医学报》2014年第9期676-678,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家863计划(2011AA10A208)

摘　　要：为调查猪嵴病毒（PKV）的流行情况,本研究根据PKV 3D基因的保守区设计了一对特异性引物,采用RT-PCR方法对2012年～2013年间从连云港地区64个规模化猪场采集的960份猪肛拭样品进行PKV检测.结果显示：PKV猪场阳性率在50％以上,个体阳性率为15.38％～90.48％;在发生腹泻的猪中PKV阳性率达46.25％,而在非腹泻的猪中阳性率为22.5％;PKV在哺乳仔猪中的阳性率为41.23％,而在育肥猪中的阳性率为22.07％.本研究调查结果为相关疫病的诊断与预防提供了依据.To investigate the prevalence of porcine kobuvirus （PKV）, a total of 960 rectal swab were collected from pigs in 64 farms of Lianyungang area during 2012 to 2013 and subjected to detection of PKV iufection in pigs using RT-PCR with a pair of primers designed according to the 3D gene of PKV. The results showed that the prevalence rate of PKV in pig farms was over 50%, and the infection rates of PKV in pigs were 15.38% to 90.48%. The detection rates of PKV in pigs with diarrhea or without diarrhea were 46.25% or 22.5%, and the positive rates in piglets or fattening pigs were 41.23% or 22.07%, respectively.

关 键 词：猪嵴病毒 腹泻 RT-PCR 

分 类 号：S852.65[农业科学—基础兽医学]