高致病性猪繁殖与呼吸综合征病毒对仔猪骨髓感染及损伤的初步研究  

Infection and lesions in bone marrow of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus

作　　者：李莉[1] 王刚[1] 刘永刚[1] 李爱东[1,3] 陶冶[1] 佟杰[1] 于颖[1,2] 涂亚斌[1] 王淑杰[1] 孙明霞[1] 姜成刚[1] 蔡雪辉[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]西北农林科技大学动物医学学院,陕西杨凌712100 [3]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国预防兽医学报》2014年第9期684-687,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31201885);公益性行业(农业)科研专项经费(201203056)

摘　　要：为研究高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)对断奶仔猪骨髓的损伤,本研究采用HP-PRRSV HuN4株接种30日龄的PRRSV抗原及抗体阴性的健康断奶仔猪,并在病毒感染后10 d检测仔猪骨髓内病毒感染及细胞凋亡情况。PCR和间接免疫荧光试验结果显示HuN4株感染仔猪骨髓内存在病毒;流式细胞计数分析和TUNEL法检测结果显示病毒感染仔猪的骨髓内出现大量的凋亡细胞。本实验结果表明,HP-PRRSV能够感染仔猪骨髓,引起仔猪骨髓损伤并导致机体免疫抑制。本研究为进一步探索HP-PRRSV感染诱导仔猪中枢免疫器官的损伤机制奠定了基础。To study the lesions in bone marrow of piglets infected with the highly pathogenic porcine reproductive and respiratory syndrome virus （HP-PRRSV）, piglets were inoculated with HuN4 strain, and the virus and apoptotic cells in bone marrow were investigated. The results showed that PRRSV was detected in bone marrow of piglets infected with HP-PRRSV by PCR and IFA, and a large number of apoptotic cells were observed in bone marrow of these piglets by flow cytometry analysis and TUNEL assay. These results demonstrated HP-PRRSV had a broader range of tissue tropism compared to classical PRRSV strains, which was able to induce the depletion of bone marrow cells, and resulted in immunosuppression. These results provided a basis for studying the lesions mechanism on the center immune organs of piglets infected with HP-PRRSV.

关 键 词：高致病性猪繁殖与呼吸综合征病毒 骨髓 细胞凋亡 

分 类 号：S852.65[农业科学—基础兽医学]