奥曲肽抑制雄激素非依赖性前列腺癌增殖的相关分子机制  

A study on molecular mechanism of octreotide inhibiting hormonal-independent prostate cancer

作　　者：徐渊[1] 杨伟[1] 殷金龙[1] 

机构地区：[1]江阴市人民医院泌尿外科,江苏省214400

出　　处：《江苏医药》2014年第17期1988-1990,共3页Jiangsu Medical Journal

摘　　要：目的探讨奥曲肽在体外抑制人雄激素非依赖性前列腺癌细胞DU145增殖作用及相关分子机制。方法以紫杉醇为对照,采用XTT法测定1000nmol/L奥曲肽对DU145细胞增殖的影响,采用人全基因表达谱芯片检测奥曲肽对DU145细胞作用前后基因表达的差异。结果奥曲肽显著抑制前列腺癌DU145细胞的增殖,抑制率(51.4±3.2)%。芯片分析结果显示,奥曲肽对DU145细胞作用前后,共有325个基因有差异表达;其中,表达量差异在2倍以上的基因58个。与增殖相关的基因p21和p27表达升高,Cyclin E和CDK2基因表达降低。结论奥曲肽能在体外抑制雄激素非依赖性前列腺癌细胞DU145的增殖;其作用机制可能与上调p21和p27基因的表达,下调Cyclin E和CDK2基因表达相关。奥曲肽有可能成为治疗雄激素非依赖性前列腺癌的药物选择。Objective To investigate the effect and mechanism of octreotide in suppressing the hormonal-independent prostate cancer cell line DU145 in vitro.Methods After treated with octreotide1000nmol/L,XTT assay was used to evaluate the cytotoxicity of octreotide on DU145.Paclitaxel 10nmol/L was used as the positive control.Affimax chips were applied to analyze the effects of octreotide on the gene expression of DU145.Results The inhibition rate of DU145 treated with octreotide 1000nmol/L for 72 h was 51.2%.Gene display analysis showed that the differential expression was found in 325 genes,of which 58 genes had a significant differential expression more than two times.The proliferation-related genes up-regulated the expressions p21 and p27and downregulated the expressions of Cyclin E and CDK2.Conclusion Octreotide may suppress the hormonalindependent prostate cancer cell line DU145 in vetro,which may be related to the up-regulation of p21 and p27and down-regulation of Cyclin E and CDK2.Octreotide is hopefully to become a choice in the treatment of hormonal-independent prostate cancer.

关 键 词：奥曲肽 雄激素非依赖性前列腺癌 DU145 

分 类 号：R737[医药卫生—肿瘤]