5,10-亚甲基四氢叶酸还原酶基因多态性与妊娠期高血压疾病的相关性研究  

Correlation between MTHFR gene polymorphism and hypertensive disorders in pregnancy

作　　者：李俊华[1] 

机构地区：[1]河北省廊坊市妇幼保健中心妇产科,河北廊坊065000

出　　处：《中国医药导报》2014年第29期11-14,共4页China Medical Herald

基　　金：河北省廊坊市科学技术研究与发展计划项目(编号2013013010C)

摘　　要：目的 探究与分析5,10-亚甲基四氢叶酸还原酶（MTHFR）基因多态性与妊娠期高血压疾病的相关性。方法选取2013年6月～2014年6月河北省廊坊市妇幼保健中心收治的60例妊娠期高血压疾病患者作为试验组,另选择同期无妊娠期高血压疾病的孕妇60例作对照组。采用荧光定量PCR技术对两组患者MTHFR C677T及A1298C基因进行检测。结果 试验组MTHFR C677T C/T基因型频率为38.33%,对照组MTHFR C677T C/T基因型频率为21.67%,试验组明显高于对照组,差异有统计学意义（χ2=4.03,P〈0.05）。试验组突变T等位基因频率为24%,对照组突变T等位基因频率为16%,试验组显著高于对照组,差异有统计学意义（χ2=4.26,P〈0.05）。试验组MTHFR A1298C A/A基因型频率为55.00%,对照组MTHFR A1298C A/A基因型频率为60.00%,组间比较差异无统计学意义（χ2=2.71,P〉0.05）。试验组MTHFR A1298C A/C基因型频率为33.33%,对照组MTHFR A1298C A/C基因型频率为26.67%,组间比较差异无统计学意义（χ2=2.66,P〉0.05）。试验组MTHFR A1298C C/C基因型频率为11.67%,对照组MTHFR A1298C C/C基因型频率为13.33%,组间比较差异无统计学意义（χ2=2.26,P〉0.05）。试验组突变A等位基因频率为76.1%,对照组突变A等位基因为79.0%,组间比较差异无统计学意义（χ2=2.09,P〉0.05）。试验组突变C等位基因频率为23.9%,对照组突变C等位基因频率为21.0%,组间比较差异无统计学意义（χ2=2.57,P〉0.05）。结论 MTHFR基因A1298C多态性与妊娠期高血压疾病的发病无明显相关性,而MTHFR基因C677T多态性可作为诱发妊娠期高血压疾病的危险因素,同时可将其作为评价妊娠期高血压疾病预后情况的一项指标,应引起临床工作者的重视。Objective To explore and analyze the correlation between 5,10-methylenetetrahydrofolate reductase （MTH- FR） gene polymorphism and hypertensive disorders in pregnancy. Methods 60 cases withgestational hypertension from June 2013 to June 2014 in Maternal and Child Health Center of Langfang City were as test group, while 60 eases of pregnant women with gestational hypertension in same period were as control group. MTHFR C677T polymorphism and A1298C gene of patients in two groups were tested by PCR-RFLP. Results In the test group MTHFR C677T C/T geno- type frequencies was 38.33%, which in the control group was 21.67%; the test group was significantly higher than that in the control group, the difference was statistically significant （X2=4.03, P. 〈 0.05）. T allele frequency of the mutation in the test group was 24%; T allele mutation in the control group was 16%, the test group was significantly higher than this in the control group, the difference was statistically significant （X2=4.26, P 〈 0.05）. MTHFR A1298C A/A genotype frequencies in the test group was 55.00%, and in the control group was 60.00%, there was no significant difference be- tween the two groups （X2=2.71, P 〉 0.05）. MTHFR A1298C A/C genotype frequency in the test group was 33.33%, and that in the eontrol group was 26.67%, there was no significant difference between the two groups （X2=2.66, P 〉 0.05）. MTHFR A1298C C/C genotype frequencies in the test group was 11.67%, and 13.33% in the control group, there was no significant difference between the two groups （X2=2.26, P 〉 0.05）. Mutant allele frequency in the test group of was76.1%, in the control group was 79.0%, there was no significant difference between the two groups （x2=2.09,P 〉 0.05）. C mutant allele frequency in the test group was 23.9%, 21.0% in the control group, there was no significant difference between the two groups （X2=2.57, P 〉 0.05）. Conclusion There is significant correlation between gene A1298C MTHFR polymorphism and the incidence of ges

关 键 词：妊娠期高血压 亚甲基四氢叶酸还原酶 基因多态性 

分 类 号：R714.246[医药卫生—妇产科学]