基于ITS2条形码序列鉴定中药材续断及其混伪品  

Identification of Dipsaci Radix and Its Adulterants Base on ITS2 Sequences

作　　者：雷美艳[1] 陈晓辰[2] 马晓冲[2] 钱齐妮[1] 竭航[1] 易思荣[1] 宋经元[1,2] 

机构地区：[1]重庆市药物种植研究所,重庆南川408435 [2]中国医学科学院北京协和医学院/药用植物研究所,北京100193

出　　处：《四川农业大学学报》2014年第3期265-269,共5页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金(81373922);重庆市科技平台与基地建设项目(cstc2012pt-gc0005)

摘　　要：【目的】利用ITS2序列对中药材续断及其混伪品进行DNA条形码鉴定,从而确保用药安全。【方法】对续断及其混伪品ITS2进行扩增并双向测序,经CodonCode Aligner V3.7.1拼接比对后,用MEGA 5.0计算种内种间K2P遗传距离,并构建NJ系统聚类树进行鉴定分析。【结果】续断药材ITS2序列比对后长度为218bp;种内K2P遗传距离分布于0-0.009 4,种间K2P遗传距离分布于0.033-0.188 2,NJ树显示续断药材及混伪品可明显区分,表现较好的单系性。【结论】ITS2序列作为DNA条形码能准确鉴别续断药材。[Objective] Internal transcribed spacers 2 （ITS2） sequences were used to identify dipsaci radix and its adulterants, which would improve its safety in clinic. [Method] ITS2 sequences of dipsaci radix and adulterants were amplified and sequenced bidireetionally. The sequence assembly and consensus sequence generation were performed by CodonCode Aligner V3.7.1. The intraspeeifie and interspeeific distances were computed and Neighbor-Joining tree was constructed by MEGA 5.0 in accordance with the Kimura 2-Parameter （K2P） mode. [Results] The length of ITS2 sequences of dipsaci radix was 218 bp. The intraspecific K2P genetic distance values ranged from 0 to 0. 009 4 and interspeeifie K2P genetic distance values ranged from 0. 033 to 0. 188 2. Neighbor-Joint tree showed that dipsaei radix differed from its adulterants obviously. [Conclusion] ITS2 may be used to identify dipsaei radix accurately.

关 键 词：续断 DNA条形码 ITS2 混伪品鉴定 

分 类 号：R931.5[医药卫生—生药学]