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作 者:张宇[1,2] 唐志鹏[1] 高兴[1] 杨培丽[1] 范琪祺 黄国弟[2]
机构地区:[1]广西大学农学院,广西南宁530004 [2]广西壮族自治区亚热带作物研究所,广西南宁530002
出 处:《经济林研究》2014年第3期72-75,共4页Non-wood Forest Research
基 金:广西自然科学基金(桂科自08320338);国家行业(农业)科研专项经费项目(201203092-5)
摘 要:为给芒果新品种的定向选育提供理论依据,以具有不同果皮颜色的8个芒果品种的叶片为试材,采用ISSR标记技术,筛选出了特异性引物,找到了与芒果果皮颜色性状相关的特异性谱带,并运用NTsys2.10软件进行了聚类分析。结果表明,利用引物UBC-864,可寻找出红色芒果品种群的特有谱带;用引物UBC-840、UBC-857、UBC-864、UBC-868和UBC-890对供试的8份芒果品种资源进行聚类分析,以相似系数0.52为标准,可将供试材料分为2类,台农1号、金煌芒、金穗芒、四季蜜芒、桂热82号聚为一类,凯特芒、爱文芒和红芒6号聚为一类。ISSR标记可用于芒果果皮颜色性状分子标记辅助育种。In order to provide a theoretical basis for breeding new cultivars of mango, taking eight mango cultivars with different peel colors as materials, some specific primers were selected out, some specifi c bands related with fruit color traits were find out by using ISSR marker techniques, and the clustering analysis was conducted with NTsys2.10 software. The results show that some specifi cspectrum bands of the red mango cultivar group can be find out by using primer UBC-864. The eight mango cultivars are cluster into two groups by the primers of UBC-840, UBC-857, UBC-864, UBC-868, UBC-890, taking similarity coefficient 0.52 as a standard. Tainong No.1, Jinhuang, Jinsui, Sijimi, Guire No.82 belong to one group, Keitt, Aiwen and Zill belong to anther group. ISSR markers can be used for molecular markerassisted breeding for fruit peel color in mango.
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