羽衣甘蓝MADS–box基因BoaAGL6的克隆及表达分析  

Cloning and expression analysis of MADS–box gene BoaAGL6 associated with floral development in kale(Brassica oleracea var. acephala)

作　　者：韩悦[1,2] 任洪杰[1,2] 翟笑雨 徐启江[1,2] 

机构地区：[1]林木遗传育种国家重点实验室,黑龙江哈尔滨150040 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《湖南农业大学学报（自然科学版）》2014年第5期487-493,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：黑龙江省自然科学基金项目(C201016);哈尔滨市科技创新人才专项资金项目(2013RFLXJ015)

摘　　要：以羽衣甘蓝(Brassica oleracea var.acephala)野生型及其在选育过程中发现的2种花瓣数量变化的突变体品种为试材,通过RACE方法克隆了AGL6基因的同源基因,命名为BoaAGL6,并用半定量RT–PCR和实时荧光定量PCR分析了BoaAGL6在3种不同花型的羽衣甘蓝各类花器官的表达模式。结果表明:克隆的BoaAGL6基因长999 bp(GenBank登录号为KC984301),开放阅读框长759 bp,编码252个氨基酸;系统发育分析表明,BoaAGL6基因属于AGL6–like进化系;半定量RT–PCR和实时定量RT–PCR结果表明,BoaAGL6基因具有较宽泛的表达区域,在不同花型的花瓣中的表达有所区别,在多瓣花型的花瓣中高丰度表达,有别于在其他2种花型中的中等水平。说明该基因在调控羽衣甘蓝花器官特征属性及形成过程中发挥重要作用。Wild type kales （Brassica oleracea var.acephala） and its two mutants which have different petals in number were used as material. One AGL6-homologue from conventional kale varieties denoted as BoaAGL6 was cloned using RACE （rapid amplification of cDNA ends）, then expression pattern of BoaAGL6 in each floral organ was examined. The results showed that BoaAGL6 contains a 759 bp open reading frame that encodes a deduced protein with 252 amino acid residues. Phylogenetic analysis indicated that BoaAGL6 belongs to the AGL6-1ike subfamily. The result from multiple methods （semi-quantitative RT-PCR and quantitative real-time PCR） revealed that the pattern of the expression of BoAGL6 is broader than those of their counterparts in eudicots, with different expression levels in petals, which is high in mutants with the most petals. These results suggest that BoaAGL6 plays important roles in controlling floral organ identity and formation in kale.

关 键 词：羽衣甘蓝 花发育 MADS–box基因 BoaAGL6基因 

分 类 号：S635.9[农业科学—蔬菜学]