参附注射液对兔供心的保护作用  

Cardioprotective effects of Shenfu Injection (SFI) added to Stanford Solution (STF) prior to heart arrest and during the cold heart preservation: an in vitro rabbit heart study

作　　者：陈伟民[1] 许少丹[1] 陈敏东[1] 陈文广[1] 黄达德[1] 

机构地区：[1]广州市第一人民医院心胸外科,510180

出　　处：《广州医药》2014年第5期10-14,共5页Guangzhou Medical Journal

基　　金：广州市卫生局中西医结合项目(2011A011002)

摘　　要：目的探讨参附注射液(SFI)能否提高供心的保护作用。方法 16只成年新西兰大白兔(雄性),在Langendorff离体灌注模型上,模拟心脏移植的"供心获取前心脏停跳"、"供心冷保存"、"主动脉开放后供心复灌"三个时期。实验组:供心离体灌注装置上平衡后,以含SFI的4℃冷Stanford液灌注诱导停跳;之后在含SFI的4℃冷Stanford液中冷浸浴原位保存4小时;保存结束后,开放主动脉重新开始灌注复苏;对照组:全程不用SFI(用生理盐水替代SFI),余同实验组。测定两组心脏血流动力学、冠脉流量、心肌梗死量、心肌组织含水量。结果使用SFI的实验组,心脏血流动力学和冠脉流量优于对照组(P<0.05),而心肌组织梗死量和心肌含水量低于对照组(P<0.05)。结论参附注射液能减轻供心"缺血-再灌注"引起的心肌损伤,显著提高供心的保护效果。Objective Previous studies have demonstrated that Shenfu injection （SFI）could protect donor heart against post-ischemia injury when applied immediately after reperfusion,but its potential cardioprotective effect added to Stanford Solu-tion （STF）prior to heart arrest and during the cold heart preservation is unknown. Methods Sixteen rabbits were anesthe-tized.Hearts were harvested and subjected to Langendorff perfusion.After equilibrium,hearts were arrested with a cold Stanford solution （STF）,followed by 4 hours of cold statistic preservation in STF,and 90 min reperfusion subsequently.SFI （60ml/L） were added to STF solution separately in above mentioned periods in experiment group （SFI-STF,n =8）,where saline vehicle were added to STF in control group （Vehicle-STF,n =8）. Results Myocardial water content and infarct size were significant-ly decreased in SFI-STF as compared with Vehicle-STF （P 〈0.05）.Cardiac function in SFI-STF hearts returned to satisfactory level shortly after the initiation of reperfusion and still better preserved throughout the reperfusion;however,vehicle-STF hearts showed persistent hypokinesia during the peri-reperfusion period. Conclusion The addition of SFI （60ml/L）to the STF in-duced an improvement in functional recovery,alleviate ischemia-reperfusion induced myocardial injury,and afford cardioprotec-tive role for donor hearts protection.

关 键 词：参附注射液 兔 供心 保护 保存 

分 类 号：R285.5[医药卫生—中药学]