Vitek 2 Compact和MicroScan WalkAway 40 SI检测MRSA的性能评价  

Performance evaluation of detecting MRSA by Vitek 2 Compact and MicroScan WalkAway 40 SI

作　　者：魏丹丹[1] 刘洋[1] 万腊根[1] 

机构地区：[1]南昌大学第一附属医院检验科,江西南昌330006

出　　处：《实验与检验医学》2014年第5期505-507,共3页Experimental and Laboratory Medicine

基　　金：江西省教育厅青年科学基金项目(编号:60513692)

摘　　要：目的评价Vitek 2 Compact和MicroScan WalkAway 40 SI两台全自动微生物鉴定仪检测耐甲氧西林金黄色葡萄球菌(MRSA)的性能。方法将临床分离的124株金黄色葡萄球菌随机分成39株和85株,分别用Vitek 2 COMPACT和MicroScan WalkAway 40 SI全自动微生物鉴定仪进行细菌鉴定及药敏分析,确认表型是否为MRSA;同时用PCR方法检测mecA及mecC基因以确定为MRSA;以PCR检测结果为金标准评价两台细菌分析仪检测MRSA的灵敏度、特异度和符合率。结果 Vitek 2 Compact和MicroScan WalkAway 40 SI检测MRSA的检出率分别为56.41%(22/39),51.76%(44/85);PCR方法检出mecA阳性菌株分别为22株、44株,均未检出mecC阳性菌株,即PCR法检测MRSA的检出率为分别为56.41%(22/39),51.76%(44/85);以PCR方法为金标准,则Vitek 2 Compact检测MRSA的灵敏度为95.45%,特异性为94.12%,符合率为94.87%;MicroScan WalkAway 40 SI检测MRSA的敏感度为93.18%,特异度为92.68%,符合率为92.94%。结论 Vitek 2COMPACT全自动微生物鉴定仪对MRSA的检出率、灵敏度、特异度和符合率均优于MicroScan WalkAway 40 SI,二者的差异无统计学意义(P>0.05),均可用于MRSA的检测。Objective To evaluate the the performance of MRSA identification between Vitek 2 Compact and MicroScan WalkAway 40 SI. Methods A total of 124 strains of Staphylococcus aureus were randomly allocated to detect whether the phenotype is MRSA or not by the two automatic microorganism identification instrument, and at the same time, detection of mecA and mecC by PCR Using the results of PCR test as the gold standard, to evaluate the sensitivity, specificity and coincidence rate of the two instruments in the detection of MRSA. Results The detection rates of MRSA by Vitek 2 Compact and MicroScan WalkAway40 SI were 56.41%（22/39） and 51.76%（44/85）, respectively, while the corresponding detection rates by PCR were 56.41%（22/39）and 51.76%（44/85）;22 and 44 mecA positive strains had been detected via the gold standard detecting method of PCR, no finding of mecC positive strains.Ultimately the sensitivity, specificity and coincidence rates of Vitek 2 Compact were 95.45%, 94.12%,94.87%, respectively, while the sensitivity, specificity and coincidence rates of MicroScan WalkAway 40 SI were 93.18%,92.68%,92.94%, respectively. Conclusions Vitek 2 COMPACT automatic microorganism identification instrument was superior to MicroScan WalkAway 40 SI in terms of the detection rate, sensitivity, specificity and coincidence rate of MRSA, but there was no significant difference between the two instruments. So both of them can be used for the detection of MRSA.

关 键 词：VITEK 2 COMPACT MICROSCAN WalkAway 40 SI MRSA 性能评价 

分 类 号：R446.5[医药卫生—诊断学] Q939.92[医药卫生—临床医学]