大豆异地衍生重组自交系群体产量相关性状的QTL定位  

Detection of soybean QTLs on yield- related traits in RIL populations derived from Peking ×7605 in two sites

作　　者：洪雪娟[1] 黄婧[1] 丁卉[1] 侯金锋[1] 李永春[1] 盖钧镒[1] 邢邯[1] 

机构地区：[1]南京农业大学大豆研究所/农业部大豆生物学与遗传育种重点实验室/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《中国油料作物学报》2014年第5期572-579,共8页Chinese Journal of Oil Crop Sciences

基　　金：大豆现代产业技术体系(CARS-004-PS10);江苏高校优势学科建设工程(PAPD);公益性行业(农业)科研专项(201303018)

摘　　要：大豆产量相关性状大多为数量性状,受环境影响较大,因此研究大豆产量相关性状的遗传及其与自然环境的关系在大豆育种中具有重要意义。以Peking×7605组合分别在济南和南京衍生的重组自交系(RIL)群体JN(RN)P7和NJ(RN)P7为材料,用146对亲本间表现多态性的SSR引物在两个群体中进行扩增,并构建遗传图谱,对大豆株高、主茎分枝数、主茎节数、单株荚数和百粒重五个产量相关性状进行考察并进行QTL分析。JN(RN)P7群体两年共检测到控制五个性状的25个QTL,NJ(RN)P7群体中共定位44个相关QTL。在JN(RN)P7群体的标记BSC和标记satt372附近两年重复检测到两个相近的QTL,NJ(RN)P7群体中两年重复检测到位于相同位置或同一置信区间的QTL共有7个。大部分QTL定位于两个RIL群体的相同连锁群上,但位置不同,并且两个群体中能够稳定表达的QTL不同。两群体中共得到了69个QTL,大多数与前人报道相符合;而由同一杂交组合在不同生态环境下衍生的两个RIL群体的QTL定位结果有所差异。Most soybean yield- related traits are quantitative and are highly susceptible to environments. Heredity study of these traits is important in breeding. Two recombinant inbred line( RIL) populations were used.They were JN( RN) P7 and NJ( RN) P7 which derived from cross Peking × 7605 at Jinan and Nanjing respectively.Two genetic linkage maps were constructed with 146 SSR primers screened in two parents and amplified in two populations. Five yield- related traits were investigated in 2005 and 2009,QTLs were analyzed with software Windows QTL Cartographer V2. 5. The yield- related traits included plant height,branches,nodes per plant,pods per plant and 100 seeds weight. A total of 25 QTLs of JN( RN) P7 population were detected,and in NJ( RN) P7 population,a total of 44 QTLs were found. Two QTLs with nearby markers BSC and satt372 were detected in both 2005 and 2009 based on JN( RN) P7 population,and seven QTLs were detected on the same position or the same confidence interval across years for NJ( RN) P7 population. Most QTLs were detected at the same linkage groups in two populations,but not the same location. The differences came from populations derived in two different sites.

关 键 词：大豆 重组自交系 异地衍生 QTL 

分 类 号：S565.103[农业科学—作物学]