辽宁花生白绢病病原鉴定及其生物学研究  

Identification of biological characteristics of Sclerotium rolfsii causing peanut stem rot in Liaoning Province

作　　者：傅俊范[1] 刘波[1] 周如军[1] 王思文[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《中国油料作物学报》2014年第5期635-640,共6页Chinese Journal of Oil Crop Sciences

基　　金：辽宁省农业攻关计划项目(2011214002)

摘　　要：对辽宁花生白绢病病原学进行了初步研究。根据病原菌形态学特征与核糖体基因内转录间隔区(rDNA-ITS)分子测序,明确辽宁花生白绢病病原菌为罗氏阿太菌Athelia rolfsii(无性世代为齐整小核菌Sclerotium rolfsii)。病原菌生物学特性研究发现菌丝的最适生长温度为30℃,温度为25℃时该菌产生的菌核最多;最适pH值为5.0;该菌虽能利用供试的7种碳源和7种氮源进行营养生长,但只在半乳糖和蔗糖作为唯一碳源的培养基中产生菌核,在尿素和赖氨酸作为唯一氮源的培养基上不产生菌核;该菌在PDA培养基上生长最好;全黑暗对菌丝生长有促进作用,但抑制菌核的产生。The pathogen causing peanut stem rot in Liaoning was researched in this project. Pathogen identification was carried out based on morphological characteristics and ribosomal DNA internal transcribed spacer( rDNA- ITS) sequences,the pathogen was identified as Athelia rolfsii( anamorph: Sclerotium rolfsii). The result of biological characteristics showed that the optimum temperature for its growth was 30℃,the optimum pH was 5. 0. All of the 7 tested carbon sources and 7 tested nitrogen sources could be used for its vegetative growth. However,A. rolfsii could only produce sclerotia on the media containing either sucrose or galactose as a carbon source,and no sclerotia were developed on the media containing either Urea or L- Lysine as a nitrogen source. The best growth was in PDA. Continuous darkness could increase the growth of hypha,but it inhibited production of sclerotia.

关 键 词：花生 白绢病 病原菌 分子鉴定 生物学特性 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]