亚麻高效再生体系的优化研究  

Optimization of high efficiency regeneration system of flax

作　　者：王玉富[1] 贾婉琪[1] 邱财生[1] 龙松华[1] 郭媛[1] 邓欣[1] 陈信波[1] 郝冬梅[1] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205

出　　处：《中国油料作物学报》2014年第5期661-666,共6页Chinese Journal of Oil Crop Sciences

基　　金：现代农业产业技术体系建设(CARS-19-E14);科技部国际科技合作与交流专项(2010DFR30620-4)

摘　　要：为了优化亚麻再生体系,对基因型、外植体的选择部位、基本培养基、植物生长调节剂等影响亚麻植株再生的因素进行了优化,建立了亚麻下胚轴高效组织培养再生体系。结果表明,亚麻种子消毒采用75%乙醇处理3min,再用0.1%升汞灭菌3min效果最好;亚麻下胚轴的下部是最佳外植体;在Y4培养基下(MS1+NAA 0.02mg/L+6-BA 1mg/L),亚麻愈伤组织的诱导率和分化率最高,分别达到98.89%和95.56%;最佳生根培养基是1/2MS+NAA 0.001mg/L,生根率达90%;同时可以看出,不同基因型再生能力不同。与派克斯和华星009相比,再生能力最强的品种是中亚2号。通过再生体系优化使亚麻愈伤组织的诱导率和分化率以及再生植株的生根率都有所提高。An efficient flax regeneration system was established through optimization of genotype,explants,basic medium and hormone combination. Results showed that surface- sterilization of hypocotyls from plant with75% ethanol for 3 min,followed by 0. 1% HgCl2 for 3 min was the optimum sterilization method. The best medium for callus induction and differentiation was Y4( MS1 + 0. 02 mg /L NAA + 1mg /L 6- BA),with an efficiency of98. 89% and 95. 56% respectively. The optimum medium for root formation of regenerated shoot was 1 /2MS +0. 001 mg /L NAA. Compared to Pinkax and Huaxing 009,Zhongya 2 had the highest regeneration efficiency. The ratio of callus induction,shoots differentiation and rooting of flax were enhanced by optimization of regeneration system.

关 键 词：亚麻 外植体 愈伤组织 再生 

分 类 号：S565.9[农业科学—作物学]