16S rDNA克隆文库法分析地下水生物反硝化系统细菌种群多样性  

Bacteria Diversity in a Denitrification-based Groundwater Remediation System by 16S rDNA

作　　者：张建美[1] 李思远[2] 高绣纺[1] 喻笑勇 时序 李根[1] 蒋兴超[1] 

机构地区：[1]长江大学地球环境与水资源学院油气资源与勘探技术教育部重点实验室,武汉430100 [2]中国地质大学(北京)水资源与环境学院,北京100083

出　　处：《科学技术与工程》2014年第30期283-288,共6页Science Technology and Engineering

基　　金：国家自然科学基金青年项目(41401545);湖北省高等学校大学生创新创业训练计划(104892013003)资助

摘　　要：采集地下水硝酸盐生物反硝化系统内的污泥样品,提取污泥样品中微生物的总DNA,构建细菌16S rDNA基因片段克隆文库,并通过16S rDNA序列系统发育分析,对反硝化系统内的细菌种群多样性以及菌群结构进行了研究。结果表明,地下水生物反硝化系统内细菌具有高度多样性,样品文库分为9个细菌类群,优势菌群为β-Proteobacteria(67.11%)和Bacteroidetes(15.79%),其中,β-proteobacteria为最优势菌群,以Rhodocyclaceae为主。对反硝化系统内细菌种群多样性的研究有利于确定优势菌种,为地下水硝酸盐生物反硝化修复奠定理论基础。Sludge sample was collected from a denitrification-based groundwater remediation system.Bacterial genome DNA was extracted,and then a bacterial 16 S rDNA genetic library was built up.After the phylogenetic analysis of 16 S rDNA sequences,bacterial community structure and diversity of the denitrification-based groundwater remediation system were studied.The results indicated that the bacterial community in the denitrification-based groundwater remediation system was highly diverse,and could be divided into nine major groups.The dominant bacterial communities in the denitrification-based groundwater remediation system were β-Proteobacteria and Bacteroidetes,which accounted for 67.11% and 15.79% of the total clones,respectively.β-Proteobacteria predominant in the ecosystem,and especially Rhodocyclaceae has absolute advantage in β-Proteobacteria clone library.The study of bacterial diversity of the denitrification-based groundwater remediation system will help to ascertain the dominant bacterial community and established a theoretical foundation for denitrification-based groundwater remediation.

关 键 词：硝酸盐 反硝化细菌 16S rDNA克隆文库法 细菌多样性 

分 类 号：X523[环境科学与工程—环境工程]