鸡CSE多克隆抗体制备及硒缺乏对鸡肝脏内源性硫化氢生成酶的影响  

Preparation of polyclonal antibody of chick CSE and the effect of selenium deficiency on endogenous hydrogen producing enzymes in chicks

作　　者：林洪金[1] 王从武[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《东北农业大学学报》2014年第9期95-99,122,共6页Journal of Northeast Agricultural University

基　　金：国家自然科学基金(31272626)

摘　　要：硒与硫化氢生成酶均在动物生理活动中起重要作用。为探讨硒缺乏对鸡内源性硫化氢生成酶的影响,在建立鸡低硒模型后,分别于15、25、35、45、55、65 d采集肝组织,应用实时荧光定量PCR法检测H2S生成酶(CSE、CBS、3-MST)mRNA表达量。结果表明,低硒可诱导CSE、CBS和3-MST mRNA表达量升高,但无时间效应,揭示H2S生成酶在硒缺乏导致的损伤中有重要作用。制备CSE多克隆抗体,为今后CSE蛋白水平研究奠定基础。Both selenium and sulfide-producing enzyme play important roles in the physiological activity of the animals. The aim of this investigate revealed the effect of generate hydrogen sulfide enzyme induced by the chicken model of selenium deficiency. Liver tissue were collected on 15, 25, 45, 55, 65 d. Real-time PCR was used to detect the expression of mRNA H2S producing enzyme （CSE, CBS, 3-MST）. The results showed that the expression level of selenium could induce the mRNA in CSE, CBS and 3-MST, but there was no effect of time, suggesting that H2S generation enzymes played an important role in the injury in selenium deficiency. At the same time, the preparation of CSE polyclonal antibody was prepared, providing a reference research on the level of CSE protein in the future.

关 键 词：硒 鸡 肝脏 硫化氢生成酶 多克隆抗体 

分 类 号：S831[农业科学—畜牧学]