绿豆环氧水解酶在硅基介孔材料上的固载及催化性能  

Immobilization of Epoxide Hydrolases from Mung Bean in Mesoporous Silicas and Their Catalytic Performance

作　　者：张艳梅[1] 洪坤强 王剑锋[1] 范圣第[1] 韩梦凡 张亚芳[1] 曹雪杰 

机构地区：[1]大连民族学院生物化工国家民委-教育部重点实验室,辽宁大连116605

出　　处：《大连民族学院学报》2014年第5期502-507,共6页Journal of Dalian Nationalities University

基　　金：国家自然科学基金项目(21203017);国家重点实验室开放基金(N1103);中央高校基本科研业务费专项资金资助项目(DC13010311);大连民族学院人才启动基金项目(20116208)

摘　　要：从绿豆中提取粗酶并优化,活性测定反应条件,结果表明,在40℃、pH=7的条件下,酶反应活性最高。将来源于绿豆的环氧水解酶固载于多种硅基介孔材料上,考察了多种因素对固定化酶活性的影响。研究发现,以大孔径、表面疏水性的材料作为载体,采用共价方法固载酶,可以得到较高的酶固载率,同时固载酶表现出较高的催化反应活性,并且可以循环使用多次。In this paper, rude epoxide hydrolase was prepared from mung beans and then the re-action conditions were optimized. The results showed that the optimum temperature is 40℃ and the optimum pH is 7. 0. Then several kinds of mesoporous silicas （MPSs） were used to immobi-lize enzyme to enhance its chemical and mechanical stabilities. The results indicated that materi-als with large pore size, hydrophobic surface gave higher immobilization efficiency and activity. Furthermore, covalent method resulted in higher stability and the solid enzyme can be reused.

关 键 词：环氧水解酶 介孔材料 固载 手性拆分 

分 类 号：Q814[生物学—生物工程]