枳实总黄酮对肝郁脾虚型大鼠肠易激综合征的治疗作用  

Pharmacodynamics Study of Total Flavonoids from Fructus Aurantii Immaturus for Treatment of Irritable Bowel Syndrome in Rats with Liver Stasis and Spleen Deficiency syndrome

作　　者：梁巧文 陈家仪[1,2] 马丽[1] 潘真真[1] 刘伟基[1] 黄校锋[1] 孙瑜梦 贾薇[1] 曾元儿[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州市药品检验所,广东广州510160

出　　处：《中药新药与临床药理》2014年第5期576-581,共6页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的探讨枳实总黄酮对肝郁脾虚型肠易激综合征(IBS)的治疗作用。方法取SD大鼠32只,随机分为正常对照组、模型组、得舒特组(1.5 mg·kg-1)和枳实总黄酮组(3 g·kg-1),每组8只,采用乙酸灌肠刺激加慢性束缚夹尾的方法,建立肝郁脾虚型IBS大鼠模型,观察第0,7,14,21,28天不同阶段大鼠的体质量、排便数、敞箱运动、内脏敏感性、糖水消耗量水平变化以及血清一氧化氮(NO)水平和结、盲肠组织中肥大细胞(MC)数目的变化。结果与正常对照组比较,模型组和给药组大鼠行为学及内脏敏感性,差异均有统计学意义(P<0.05,P<0.01),表现为应激21 d,体质量增长缓慢,排便数逐渐增加,模型组和治疗组大鼠于第7,14,21,28 d排便数均高于相应阶段的正常对照组;糖水吸收量在不同阶段有波动,但模型复制21 d后,模型组、得舒特组、枳实总黄酮组大鼠蔗糖偏嗜度均低于正常对照组;内脏敏感性显著增加。给药治疗1周后,枳实总黄酮组和得舒特组大鼠排便数减少、糖水消耗量增加、活动增加、内脏敏感性降低;枳实总黄酮能提高模型大鼠血清NO的含量,并且减少模型大鼠结盲肠组织MC的数目。结论枳实总黄酮对肝郁脾虚型IBS大鼠具有治疗作用。Objective To investigate the therapeutic effect of total flavonoids from Fructus Aurantii Immaturus （FAI-TF） for irritable bowel syndrome （IBS） in rats with liver stasis and spleen deficiency syndrome. Methods Thirty-two SD rats were randomized into four groups, normal control group, model group, Dicete group（1.5 mg·kg^-1）, and FAI-TF group （6 g·kg^-1）, 8 rats in each group. IBS model was established by giving an enema of acetic acid and by chronic tail-clamping stress. On experimental day O, 7, 14, 21, 28, rat body mass, the number of defecation grains, behaviors in the open field, abdominal withdrawal reflex, and sucrose intake were observed. Nitric oxide（NO） content in the serum and the number of mast cells （MC） of rat colon and ileum were simultaneously measured. Results Compared with the normal control group, rat behavior and visceral sensitivity in the model group and medication group were significantly different （P 〈 0.05, P 〈 0.01） , which expressed as slow growth of body mass during the modeling period of 21 days, and significant increase of rat defecation and visceral sensitivity on experimental day 7, 14, 21, 28. Sucrose intake volume fluctuated with 21 days, and then the volume was less in the model group and medication groups than that in the normal control group. After medication for one week, Dicete groupand FAI-TF group had less defecation granules, larger sucrose intake volume, more active behaviors and lower visceral sensitivity than the model group. FAI-TF group had an effect on increasing the serum NO content and reducing MC count in the colon and ileum of model rat. Conclusion Total flavonoids from Fructus Aurantii Immaturus have certain therapeutic effect for IBS in rats with liver stasis and spleen deficiency syndrome.

关 键 词：枳实总黄酮 肝郁脾虚 肠易激综合征 一氧化氮 肥大细胞 

分 类 号：R285.5[医药卫生—中药学]